SU-DHL-1 (ATCC® CRL-2955™)

Organism: Homo sapiens, human  /  Tissue: peritoneal effusion  /  Cell Type: histiocytic

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Homo sapiens, human
Tissue peritoneal effusion
Cell Type histiocytic
Morphology lymphoblast-like
Culture Properties suspension
Biosafety Level 1
Disease large cell lymphoma; diffuse histiocytic lymphoma
Age 10 years
Gender Male
Ethnicity Caucasian
Applications The availability of a permanently established human neoplastic cell line as the source of this type C RNA virus should greatly facilitate studies of its biological significance. Evidence has recently been obtained that the virus can induce proliferation of normal human monocytes and histiocytes in vitro.
Storage Conditions liquid nitrogen vapor phase
Karyotype 88 chromosomes; t(2;5)(p23;q35) translocation
Clinical Data 10 years
Caucasian
Male
Antigen Expression Antigen Expression:
Monocyte Marker: CD163+
Lymphoid Marker: CD45-
Progenitor Markers: CD10-, CD34-
Activation Markers: CD30+, CD25+, CD70+, CD71+, CD80-, HLA-DR+, CD45-
T-Cell Markers: CD2-, CD3-, CD4-, CD5+, CD7-, CD8-
B-Cell Markers: CD19-, CD20-, CD21-, CD22-
Myelomonocytic Markers: CD11b-, CD11c-, CD13-, CD14-, CD15-, CD33-
Markers Cytochemical Analysis:
PAS +++ (Periodic Acid Schiff reaction)
PAScD - (Periodic Acid Schiff reaction with diastase digestion)
SBB+ (Sudan Black B)
Oil Red O +++
Peroxidase -
AcP +++ (acid phosphatase)
AcPcT - (acid phosphatase with tartrate)
AlkP- (alkaline phosphatase)
NA +++ (alpha-naphthyl acetate esterase)
NASDA +++ ( Naphtol AS-D acetate esterase)
NASDAF +++ (Naphtol AS-D acetate esterase with NaF inhibition)
NCA - ( Naphtol AS-D chloroacetate esterase)
gl ++ (beta-glucuronidase)
LAP - ( Leucocyte alkaline phosphatase)
Genes Expressed Fusion gene NPM-ALK (p80)+
anaplastic lymphoma kinase (ALK)+

Ig, not expressed
Comments The cells are surface Ig negative (sIG-).
The cells are non-specific esterase, acid phosphatase and oil red O positive.
The cells are periodic acid Schiff negative.
These cells phagocytose Candida albicans and latex particles.
The cells are reported to be very weakly E - rosette positive.
ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Protocol: Cultures can be maintained by the addition of fresh medium. An inoculum of 8.0 x 104to 2.0 x 105cells/mL is recommended. Subculture when the cell concentration is between 1.0 x 106 and 1.5 x 106 cells/mL.
Subcultivation ratio: A subcultivation ratio of 1:4 to 1:12 is recommended.
Interval: As needed.
Medium renewal: every 2 to 4 days
Cryopreservation Storage temperature: liquid nitrogen vapor phase
Freeze medium: Complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO
Culture Conditions Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile D5S818: 11, 12
D13S317: 9, 13
D7S820: 10, 13
D16S539: 11, 12
vWA: 15, 17
THO1: 6, 7
Amelogenin: X Y
TPOX: 8
CSF1PO: 12
Population Doubling Capacity infinite
Name of Depositor A Epstein
References

Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220

Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039

Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
References

Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220

Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039

Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794