A431NS (ATCC® CRL-2592)

Organism: Homo sapiens, human  /  Tissue: skin/epidermis  /  Disease: epidermoid carcinoma

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Organism Homo sapiens, human
Tissue skin/epidermis
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease epidermoid carcinoma
Age 85 years
Gender female
Applications
While cell sensitivity to drugs tend to be changeable, the A431NS cell line has a stable sensitivity to LIC. The cell line can be used to test the antiproliferation activity of LIC.
Storage Conditions liquid nitrogen vapor temperture
Derivation
A431NS was derived from the A431 cell line (ATCC CRL-1555) in 1997 by repeated subculturing to select cells that detached from the substrate easily.
Clinical Data
female
85 years
Comments

The polyinosinic-polycytidylic acid/cationic liposome complex (LIC) inhibits cancer cell growth by the induction of apoptosis.

LIC has strong cytotoxic effects on the A431NS cell line but neither polyinosinic-polycytidylic acid alone nor cationic liposome alone have antiproliferation effects.

Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.   
  2. Briefly rinse the cell layer with PBS to remove all traces of serum which contains trypsin inhibitor.   
  3. Remove the PBS completely and add 2.0 to 3.0 ml of  0.25% (w/v) Trypsin-053mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually with 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium containing 10% FBS and aspirate cells by gently pipetting.   
  5. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes.    
  6. Discard supernatant and resuspend cells in fresh culture medium.  Add appropriate aliquots of cell suspension to new culture vessels.
  7. Place culture vessels in incubators at 37°C. 

Subcultivation Ratio:  1:3 to 1:6.
Medium Renewal: Every 2 to 3 days.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994

Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperture
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 9,13
D16S539: 12,14
D5S818: 12,13
D7S820: 10
THO1: 9
TPOX: 11
vWA: 15,17
Name of Depositor K Hirabayashi
References

Hirabayashi K, et al. Inhibition of cancer cell growth by polyinosinic-polycytidylic acid/cationic liposome complex: a new biological activity. Cancer Res. 59: 4325-4333, 1999. PubMed: 10485480

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Hirabayashi K, et al. Inhibition of cancer cell growth by polyinosinic-polycytidylic acid/cationic liposome complex: a new biological activity. Cancer Res. 59: 4325-4333, 1999. PubMed: 10485480