TK-1 (ATCC® CRL-2396)

Organism: Mus musculus, mouse  /  Cell Type: T lymphocyte  /  Disease: lymphoma

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Organism Mus musculus, mouse
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease lymphoma
Strain AKR/Cum
Applications
TK1 cells have a unique phenotype with high levels of the lymphocyte integrin, alpha 4, beta 7. TK1 cells are useful for studies of alpha 4, beta 7 function and regulation.
Storage Conditions liquid nitrogen vapor phase
Derivation
TK1 is a T-cell lymphoma cell line originated by E. C. Butcher and I. L. Weissman from a spontaneous thymic lymphoma/leukemia that developed in an AKR/Cum mouse.
 RefButcher EC, et al. Organ specificity of lymphocyte migration: mediation by highly selective lymphocyte interaction with organ-specific determinants on high endothelial venules. Eur. J. Immunol. 10: 556-561, 1980. PubMed: 6157544
Antigen Expression
CD8 +; alpha4, beta7 integrin +
Comments They bind constitutively to the murine mucosal vascular addressin, MAdCAM-1. 

TK1 cells have a unique phenotype with high levels of the lymphocyte integrin, alpha 4, beta 7. 

They bind constitutively to the murine mucosal vascular addressin, MAdCAM-1. RefBerlin C, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74: 185-195, 1993. PubMed: 7687523 

The cells are CD8 positive; they display approximately 1% of L-selectin of that expressed by normal lymph node cells. 

Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.1 mM non-essential amino acids, 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 1 to 2 x 105 viable cells/mL. Do not allow the cell concentration to exceed 2 x 106 cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Name of Depositor EC Butcher
References

Berlin C, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74: 185-195, 1993. PubMed: 7687523

Berlin C, et al. alpha 4 integrins mediate lymphocyte attachment and rolling under physiologic flow. Cell 80: 413-422, 1995. PubMed: 7532110

Butcher EC, et al. Organ specificity of lymphocyte migration: mediation by highly selective lymphocyte interaction with organ-specific determinants on high endothelial venules. Eur. J. Immunol. 10: 556-561, 1980. PubMed: 6157544

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Berlin C, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74: 185-195, 1993. PubMed: 7687523

Berlin C, et al. alpha 4 integrins mediate lymphocyte attachment and rolling under physiologic flow. Cell 80: 413-422, 1995. PubMed: 7532110

Butcher EC, et al. Organ specificity of lymphocyte migration: mediation by highly selective lymphocyte interaction with organ-specific determinants on high endothelial venules. Eur. J. Immunol. 10: 556-561, 1980. PubMed: 6157544