803-15.6 (ATCC® CRL-2395)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with both native and denatured HIV-1 gp120 within the conserved C-terminal amino acid sequence residues 495-516.
The antibody can be used for the purification of gp120 using a milder elution condition (pH4) than many other antibodies; it can be used in ELISA and Western Blot assays.
Derivation
Animals were immunized with recombinant immunodeficiency virus 1 (HIV-1) gp120 (IIIB variant) purified from Baculovirus supernatant.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human immunodeficiency virus 1 (HIV-1) gp120.
Cellular Products
immunoglobulin; monoclonal antibody; against human immunodeficiency virus 1 (HIV-1) gp120.
Comments
Animals were immunized with recombinant immunodeficiency virus 1 (HIV-1) gp120 (IIIB variant) purified from Baculovirus supernatant.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The cell line was subcloned six times and adapted to growth in serum-free medium.
The antibody reacts with both native and denatured HIV-1 gp120 within the conserved C-terminal amino acid sequence residues 495-516.
The antibody can be used for the purification of gp120 using a milder elution condition (pH4) than many other antibodies; it can be used in ELISA and Western Blot assays.
Complete Growth Medium HB101 medium supplemented with 2 mM L-glutamine, 1.0 mM sodium pyruvate, 10 mM HEPES and 0.021 mM 2-mercaptoethanol
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
Culture medium, 75%; fetal bovine serum, 20%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG1
Name of Depositor MR van Schravendijk
References

Golden A, et al. Effect of promoters and signal sequences on the production of secreted HIV-1 gp120 protein in the baculovirus system. Protein Expr. Purif. 14: 8-12, 1998. PubMed: 9758745

Ferrer M, et al. Construction and characterization of a radio-iodinatable mutant of recombinant human CD4. J. Immunol. Methods 210: 215-225, 1997. PubMed: 9520304

Ferrer M, Harrison SC. Peptide ligands to human immunodeficiency virus type 1 gp120 identified from phage display libraries. J. Virol. 73: 5795-5802, 1999. PubMed: 10364331

Ferrer M, et al. Structural and functional characterization of an epitope in the conserved C-terminal region of HIV-1 gp120. J. Peptide Res. 54: 32-42, 1999. PubMed: 10448968

Basic Documentation
References

Golden A, et al. Effect of promoters and signal sequences on the production of secreted HIV-1 gp120 protein in the baculovirus system. Protein Expr. Purif. 14: 8-12, 1998. PubMed: 9758745

Ferrer M, et al. Construction and characterization of a radio-iodinatable mutant of recombinant human CD4. J. Immunol. Methods 210: 215-225, 1997. PubMed: 9520304

Ferrer M, Harrison SC. Peptide ligands to human immunodeficiency virus type 1 gp120 identified from phage display libraries. J. Virol. 73: 5795-5802, 1999. PubMed: 10364331

Ferrer M, et al. Structural and functional characterization of an epitope in the conserved C-terminal region of HIV-1 gp120. J. Peptide Res. 54: 32-42, 1999. PubMed: 10448968