803-15.6 (ATCC® CRL-2395)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with both native and denatured HIV-1 gp120 within the conserved C-terminal amino acid sequence residues 495-516.
The antibody can be used for the purification of gp120 using a milder elution condition (pH4) than many other antibodies; it can be used in ELISA and Western Blot assays.
Derivation
Animals were immunized with recombinant immunodeficiency virus 1 (HIV-1) gp120 (IIIB variant) purified from Baculovirus supernatant.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human immunodeficiency virus 1 (HIV-1) gp120.
Cellular Products
immunoglobulin; monoclonal antibody; against human immunodeficiency virus 1 (HIV-1) gp120.
Comments
Animals were immunized with recombinant immunodeficiency virus 1 (HIV-1) gp120 (IIIB variant) purified from Baculovirus supernatant.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The cell line was subcloned six times and adapted to growth in serum-free medium.
The antibody reacts with both native and denatured HIV-1 gp120 within the conserved C-terminal amino acid sequence residues 495-516.
The antibody can be used for the purification of gp120 using a milder elution condition (pH4) than many other antibodies; it can be used in ELISA and Western Blot assays.
Complete Growth Medium HB101 medium supplemented with 2 mM L-glutamine, 1.0 mM sodium pyruvate, 10 mM HEPES and 0.021 mM 2-mercaptoethanol
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
Culture medium, 75%; fetal bovine serum, 20%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG1
Name of Depositor MR van Schravendijk
References

Golden A, et al. Effect of promoters and signal sequences on the production of secreted HIV-1 gp120 protein in the baculovirus system. Protein Expr. Purif. 14: 8-12, 1998. PubMed: 9758745

Ferrer M, et al. Construction and characterization of a radio-iodinatable mutant of recombinant human CD4. J. Immunol. Methods 210: 215-225, 1997. PubMed: 9520304

Ferrer M, Harrison SC. Peptide ligands to human immunodeficiency virus type 1 gp120 identified from phage display libraries. J. Virol. 73: 5795-5802, 1999. PubMed: 10364331

Ferrer M, et al. Structural and functional characterization of an epitope in the conserved C-terminal region of HIV-1 gp120. J. Peptide Res. 54: 32-42, 1999. PubMed: 10448968

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Golden A, et al. Effect of promoters and signal sequences on the production of secreted HIV-1 gp120 protein in the baculovirus system. Protein Expr. Purif. 14: 8-12, 1998. PubMed: 9758745

Ferrer M, et al. Construction and characterization of a radio-iodinatable mutant of recombinant human CD4. J. Immunol. Methods 210: 215-225, 1997. PubMed: 9520304

Ferrer M, Harrison SC. Peptide ligands to human immunodeficiency virus type 1 gp120 identified from phage display libraries. J. Virol. 73: 5795-5802, 1999. PubMed: 10364331

Ferrer M, et al. Structural and functional characterization of an epitope in the conserved C-terminal region of HIV-1 gp120. J. Peptide Res. 54: 32-42, 1999. PubMed: 10448968