SVR (SVEN 1 ras) (ATCC® CRL-2280™)

Organism: Mus musculus, mouse  /  Cell Type: SV40 transformed

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Mus musculus, mouse
Cell Type SV40 transformed
Product Format frozen
Culture Properties adherent
Biosafety Level 2 Cells contain polyomavirus DNA sequences
Strain C57BL/6
Applications
The tumors in nude mice cause death by hemorrhage and anemia with thrombocytopenia, and can be used to test angiogenesis inhibitors.
Derivation
SVR is a derivative of MS1, a pancreatic islet endothelial cell line (see ATCC CRL-2279).
Markers
vascular endothelial growth factor (VEGF)
Genes Expressed
tissue inhibitor of bioreactive matrix metalloproteinase (high levels)
Tumorigenic Yes
Comments
SVR is a derivative of MS1, a pancreatic islet endothelial cell line (see ATCC CRL-2279).
MS1 cells were transduced with a retrovirus encoding H-ras and hygromycin resistance.
The tumors in nude mice cause death by hemorrhage and anemia with thrombocytopenia, and can be used to test angiogenesis inhibitors.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.
Add fresh trypsin solution (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor JL Arbiser
References

Arbiser JL, et al. Oncogenic H-ras stimulates tumor angiogenesis by two distinct pathways. Proc. Natl. Acad. Sci. USA 94: 861-866, 1997. PubMed: 9023347

Tsou R, et al. Retroviral delivery of dominant-negative vascular endothelial growth factor receptor type 2 to murine wounds inhibits wound angiogenesis. Wound Repair Regen. 10: 222-229, 2002. PubMed: 12191004

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
References

Arbiser JL, et al. Oncogenic H-ras stimulates tumor angiogenesis by two distinct pathways. Proc. Natl. Acad. Sci. USA 94: 861-866, 1997. PubMed: 9023347

Tsou R, et al. Retroviral delivery of dominant-negative vascular endothelial growth factor receptor type 2 to murine wounds inhibits wound angiogenesis. Wound Repair Regen. 10: 222-229, 2002. PubMed: 12191004