RAW 264.7 gamma NO(-) (ATCC® CRL-2278)

Organism: Mus musculus, mouse  /  Cell Type: Monocyte, Macrophage  / 

Organism Mus musculus, mouse
Cell Type Monocyte, Macrophage
Product Format frozen
Culture Properties adherent
Biosafety Level 2  [Cells contain Abelson Murine Leukemia Virus viral DNA]
Gender male
Strain BALB/c
Storage Conditions liquid nitrogen vapor phase
Derivation
RAW 264.7 gamma NO(-) was derived from the RAW 264.7 (see ATCC TIB-71) mouse monocyte/macrophage cell line ordinally obtained in 1978 from Dr. Peter Ralph.
The cells were not intentionally cloned but were serendipitously obtained during routine culture.
Clinical Data
male
Antigen Expression
H-2d
Receptor Expression
complement (C3), expressed
Genes Expressed
lysozyme
Comments

Unlike the parental line, RAW 264.7 gamma NO(-) does not produce nitric oxide upon treatment with interferon gamma alone, but requires LPS for full activation (the iNOS promoter linked to a luciferase reporter gene is also unresponsive to IFN- alone).

This property makes its behavior more like that of normal macrophages from some commonly used strains of mice.

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cells can be grown as a monolayer or in spinner cultures. Routine passage cells should be grown on bacterial grade culture dishes. Subcultures are prepared by scraping cells from floor of dishes every two days and diluting to 1 x 106 cells/20 mL (3 x 105 cells/20 mL for weekend passage). Cells grown in spinner flasks are inoculated at a density of 1 x 105 cells/mL. Spinner culture cell densities should not be allowed to rise above 6 x 105 cells/mL.

Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Name of Depositor SW Russell
Year of Origin 1978
References

Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031

Raschke WC, et al. Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198

Alley EW, et al. A classical enhancer element responsive to both lipopolysaccharide and interferon-gamma augments induction of the iNOS gene in mouse macrophages. Gene 158: 247-251, 1995. PubMed: 7541763

Lorsbach RB, et al. Expression of the nitric oxide synthase gene in mouse macrophages activated for tumor cell killing. Molecular basis for the synergy between interferon-gamma and lipopolysaccharide. J. Biol. Chem. 268: 1908-1912, 1993. PubMed: 7678412

Basic Documentation
References

Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031

Raschke WC, et al. Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198

Alley EW, et al. A classical enhancer element responsive to both lipopolysaccharide and interferon-gamma augments induction of the iNOS gene in mouse macrophages. Gene 158: 247-251, 1995. PubMed: 7541763

Lorsbach RB, et al. Expression of the nitric oxide synthase gene in mouse macrophages activated for tumor cell killing. Molecular basis for the synergy between interferon-gamma and lipopolysaccharide. J. Biol. Chem. 268: 1908-1912, 1993. PubMed: 7678412