X22 (ATCC® CRL-2228)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with human, rodent and bovine clathrin, and is useful for immunofluorescence and immunoprecipitation applications.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human clathrin heavy chain
Cellular Products
immunoglobulin; monoclonal antibody; against human clathrin heavy chain
Comments
Animals were immunized with human brain clathrin.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with human, rodent and bovine clathrin, and is useful for immunofluorescence and immunoprecipitation applications.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 15%

  • This medium is formulated for use with a 5% CO2 in air atmosphere. (Standard DMEM formulations contain 3.7 g/L sodium bicarbonate and a 10% CO2 in air atmosphere is then recommended).
    Subculturing
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 0.5 X 10 exp5 to 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
    Cryopreservation
    Culture medium, 95%; DMSO, 5%
    Isotype IgG1
    Name of Depositor FM Brodsky
    References

    Brodsky FM. Clathrin structure characterized with monoclonal antibodies. I. Analysis of multiple antigenic sites. J. Cell Biol. 101: 2047-2054, 1985. PubMed: 2415533

    Basic Documentation
    Restrictions

    Note: These cells are distributed for research purposes only subject to the following: 1) The X22 cell line or its products must not be distributed to third parties. Commercial interests are the exclusive property of Frances M. Brodsky and Becton Dickinson Immunocytometry Systems. 2) Any proposed commercial use of these cells or their products must first be negotiated with Frances M. Brodsky and Becton Dickinson Immunocytometry Systems. 3) In all papers reporting any use of these cells or derived products, a direct reference will be made to J. Cell Biol. 101:2047-2054, 1985

    References

    Brodsky FM. Clathrin structure characterized with monoclonal antibodies. I. Analysis of multiple antigenic sites. J. Cell Biol. 101: 2047-2054, 1985. PubMed: 2415533