B104-1-1 (ATCC® CRL-1887)

Organism: Mus musculus, mouse  /  Cell Type: neuroblast,Neuroblastoma,Glioblastoma  /  Disease: Neuroblastoma,Glioblastoma

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Organism Mus musculus, mouse
Cell Type neuroblast,Neuroblastoma,Glioblastoma
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease Neuroblastoma,Glioblastoma
Age embryo
Strain NIH/Swiss
Applications
This line was established by A.L. Schechter et al.
Derivation
This line was established by A.L. Schechter et al. in 1984 by transfecting NIH/3T3 cells with EcoR1 digested DNA from the rat neuroblastoma cell line B-104.
Comments
This line was established by A.L. Schechter et al. in 1984 by transfecting NIH/3T3 cells with EcoR1 digested DNA from the rat neuroblastoma cell line B-104.
The cells contain the neu transforming gene which codes for a 185000 dalton antigen designated p185.
The p185 protein is strongly associated with the presence of glioblastoma and neuroblastoma oncogenes.
The neu oncogene is homologous to the erb-B oncogene, and p185 is serologically similar to the epidermal growth factor receptor.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose; 90%; bovine calf serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:100 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin, 0.03% EDTA, rinse and remove trypsin and let the culture sit at 37C until the cells detach (5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture twice weekly.
Name of Depositor RA Weinberg
Year of Origin 1984
References

Schechter AL, et al. The neu oncogene: an erb-B-related gene encoding a 185,000-Mr tumour antigen. Nature 312: 513-516, 1984. PubMed: 6095109

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These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Schechter AL, et al. The neu oncogene: an erb-B-related gene encoding a 185,000-Mr tumour antigen. Nature 312: 513-516, 1984. PubMed: 6095109