PC-12 (ATCC® CRL-1721)

Organism: Rattus norvegicus, rat  /  Tissue: adrenal gland  /  Disease: pheochromocytoma

Organism Rattus norvegicus, rat
Tissue adrenal gland
Product Format frozen
Morphology small irregularly shaped cells
Culture Properties floating clusters; few scattered lightly attached cells.
Biosafety Level 1
Disease pheochromocytoma
Gender male
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype 40 chromosomes; 38 autosomes plus XY RefGreene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897
Images
Derivation
The PC-12 cell line was derived from a transplantable rat pheochromocytoma.
Clinical Data
male
Receptor Expression
nerve growth factor (NGF), expressed
Genes Expressed
catecholamines; dopamine; norepinephrine
Cellular Products
catecholamines; dopamine; norepinephrine
Tumorigenic Yes
Effects
Yes, in New England Deaconess Hospital strain rats
Comments
The cells respond reversibly to NGF by induction of the neuronal phenotype when plated on Collagen IV coated culture flasks.
The cells do not synthesize epinephrine.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
  • heat-inactivated horse serum to a final concentration of 10%
  • fetal bovine serum to a final concentration of 5%

Subculturing
Protocol: Volumes used for this protocol are for a 75cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product.
  • Transfer cell suspension to centrifuge tube. Centrifuge cells at 180 to 225 xg for 8-15 minutes at room temperature.
  • Remove and discard supernatant leaving cell pellet.
  • Resuspend the cell pellet with 5 mls of fresh medium (or use an appropriate volume of medium which is a multiple of 5 to facilitate the next step).
  • Gently aspirate each 5 ml aliquot of cells 4 or 5 times with a new 20 ml syringe outfitted with a 22g (1½ in.) needle to break up cell clusters.
  • Add appropriate aliquots of the cell suspension to new 75 cm2 flask with 10-15 ml fresh growth medium. Seed flask 5 x 10(5) to 1 x 10(6) viable cells/ml or use subcultivation ratio of 1:2 to 1:4.
  • Place culture vessels in incubator at 37ºC Subculture when cell density reaches between 2-4 x 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Population Doubling Time 48 hrs
Name of Depositor B Patterson
References

Levi A, et al. Molecular cloning of a gene sequence regulated by nerve growth factor. Science 229: 393-395, 1985. PubMed: 3839317

Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897

Biocca S, et al. A macromolecular structure favouring microtubule assembly in NGF- differentiated pheochromocytoma cells (PC12). EMBO J. 2: 643-648, 1983. PubMed: 6641712

Weber E, et al. Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells. J. Biol. Chem. 271: 6963-6971, 1996. PubMed: 8636125

U.S. Pharmacopeia USP Monographs: Small Intestinal Submucosa Wound Matrix. Rockville, MD. USP32-NF27, 2005

Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897

Cross References

Nucleotide (GenBank) : AF311055 Rattus norvegicus thioredoxin mRNA, complete cds.

Nucleotide (GenBank) : AF319950 Rattus norvegicus glutaredoxin mRNA, complete cds.

Nucleotide (GenBank) : AF311054 Rattus norvegicus APEX (Apex) mRNA, partial cds, alternatively spliced.

Basic Documentation
Other Documentation
FAQ's
  1. PC-12 Cells: ATCC® CRL-1721 vs. ATCC® CRL-1721.1


    Date Updated: 7/1/2014

  2. Coating flasks with Collagen IV for CRL-1721 differentiation


    Date Updated: 7/1/2014

References

Levi A, et al. Molecular cloning of a gene sequence regulated by nerve growth factor. Science 229: 393-395, 1985. PubMed: 3839317

Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897

Biocca S, et al. A macromolecular structure favouring microtubule assembly in NGF- differentiated pheochromocytoma cells (PC12). EMBO J. 2: 643-648, 1983. PubMed: 6641712

Weber E, et al. Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells. J. Biol. Chem. 271: 6963-6971, 1996. PubMed: 8636125

U.S. Pharmacopeia USP Monographs: Small Intestinal Submucosa Wound Matrix. Rockville, MD. USP32-NF27, 2005

Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897