HT-1376 (ATCC® CRL-1472)

Organism: Homo sapiens, human  /  Tissue: urinary bladder  /  Disease: grade 3, carcinoma

Organism Homo sapiens, human
Tissue
urinary bladder
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease grade 3, carcinoma
Age 58 years
Gender female
Ethnicity Caucasian
Applications This cell line is a suitable transfection host.
Karyotype modal chromosome number = 104 to 121
Clinical Data
58 years
Caucasian
female
Genes Expressed
fibrinolytic activity; interferon
Cellular Products
fibrinolytic activity; interferon
Tumorigenic Yes
Effects
Yes, in mice and hamsters
Comments
The line does not contain detectable virus particles, herpesvirus antigens or retrovirus antigens.
The cells will grow in soft agar.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution, rinse and remove. Allow culture to sit at room temperature or 37°C until cells detach (about 10 minutes), add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 9,11
D16S539: 11,14
D5S818: 11,12
D7S820: 9,12
THO1: 7,10
TPOX: 8
vWA: 15,18
Isoenzymes
G6PD, B
Name of Depositor S Rasheed
References

Rasheed S, et al. Human bladder carcinoma: characterization of two new tumor cell lines and search for tumor viruses. J. Natl. Cancer Inst. 58: 881-890, 1977. PubMed: 191628

Jameson P, Grossberg SE. Production of interferon by human tumor cell lines. Arch. Virol. 62: 209-219, 1979. PubMed: 229805

Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484

Basic Documentation
Other Documentation
References

Rasheed S, et al. Human bladder carcinoma: characterization of two new tumor cell lines and search for tumor viruses. J. Natl. Cancer Inst. 58: 881-890, 1977. PubMed: 191628

Jameson P, Grossberg SE. Production of interferon by human tumor cell lines. Arch. Virol. 62: 209-219, 1979. PubMed: 229805

Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484