CHO-K1 (ATCC® CCL-61)

Organism: Cricetulus griseus, hamster, Chinese  /  Tissue: ovary  / 

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Organism Cricetulus griseus, hamster, Chinese
Tissue ovary
Product Format frozen
Morphology epithelial-like
Culture Properties adherent
Biosafety Level 1
Gender female
Applications
This cell line is suitable as a transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype Chromosome Frequency Distribution 50 Cells: 2n = 22. Stemline number is hypodiploid.
Images
Derivation
The CHO-K1 cell line was derived as a subclone from the parental CHO cell line initiated from a biopsy of an ovary of an adult Chinese hamster by T. T. Puck in 1957.
Clinical Data
female
Virus Susceptibility Vesicular stomatitis, Orsay (Indiana)
Vesicular stomatitis, Glasgow (Indiana)
Getah virus
Virus Resistance
poliovirus 2; modoc virus; Button Willow virus
Comments

The cells require proline in the medium for growth.

Complete Growth Medium The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
    3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
    4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
    5. Add appropriate aliquots of the cell suspension to new culture vessels.
    6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Once or twice between subculture
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Name of Depositor TT Puck
Year of Origin 1957
References

Puck TT, et al. Genetics of somatic mammalian cells III. Long-term cultivation of euploid cells from human and animal subjects. J. Exp. Med. 108: 945-956, 1958. PubMed: 13598821

Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc. Natl. Acad. Sci. USA 53: 288-293, 1965. PubMed: 14294058

Kao FT, Puck TT. Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells. Proc. Natl. Acad. Sci. USA 60: 1275-1281, 1968. PubMed: 5244736

Kao FT, Puck TT. Genetics of somatic mammalian cells. IV. Properties of Chinese hamster cell mutants with respect to the requirement for proline. Genetics 55: 513-524, 1967. PubMed: 6068403

Kao FT, et al. Complementation analysis on virus-fused Chinese hamster cells with nutritional markers. Science 164: 312-314, 1969. PubMed: 5813351

Kao F, et al. Genetics of somatic mammalian cells. X. Complementation analysis of glycine-requiring mutants. Proc. Natl. Acad. Sci. USA 64: 1284-1291, 1969. PubMed: 5271752

Mayr GA, Freimuth P. A single locus on human chromosome 21 directs the expression of a receptor for adenovirus type 2 in mouse A9 cells. J. Virol. 71: 412-418, 1997. PubMed: 8985365

Hoppe HC, et al. Identification of phosphatidylinositol mannoside as a mycobacterial adhesin mediating both direct and opsonic binding to nonphagocytic mammalian cells. Infect. Immun. 65: 3896-3905, 1997. PubMed: 9284169

Aragon V, et al. Effect of cytolethal distending toxin on F-actin assembly and cell division in Chinese hamster ovary cells. Infect. Immun. 65: 3774-3780, 1997. PubMed: 9284151

Yang TT, et al. Quantification of gene expression with a secreted alkaline phosphatase reporter system. BioTechniques 23: 1110-1114, 1997. PubMed: 9421645

St. Geme JW, et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830

Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260

Siess DC, et al. Exceptional fusogenicity of chinese hamster ovary cells with murine retrovirus suggests roles for cellular factor(s) and receptor clusters in the membrane fusion process. J. Virol. 70: 3432-439, 1996. PubMed: 8648675

Wang H, et al. Modulation of ecotropic murine retrovirus by N-linked glycosylation of the cell surface receptor/amino acid transporter. J. Virol. 70: 6884-6891, 1996. PubMed: 8794331

Chen Y, et al. Demonstration of binding of dengue virus envelope protein to target cells. J. Virol. 70: 8765-8772, 1996. PubMed: 8971005

Nohturfft A, et al. Recurrent G-to-A substitution in a single codon of SREBP cleavage-activating protein causes sterol resistance in three mutant chinese hamster ovary cell lines. Proc. Natl. Acad. Sci. USA 93: 13709-13714, 1996. PubMed: 8942999

Blanke SR, et al. Fused polycationic peptide mediates delivery on diphtheria toxin A chain to the cytosol in the presence of anthrax protective antigen. Proc. Natl. Acad. Sci. USA 93: 8437-8442, 1996. PubMed: 8710889

Efthimiopoulos S, et al. Cholinergic agonists stimulate secretion of soluble full-length amyloid precursor protein in neuroendocrine cells. Proc. Natl. Acad. Sci. USA 93: 8046-8050, 1996. PubMed: 8755600

Sharma A, et al. Reduction in the level of Gal(alpha 1,3)Gal in transgenic mice and pigs by the expression of an alpha(1,2)fucosyltransferase. Proc. Natl. Acad. Sci. USA 93: 7190-7195, 1996. PubMed: 8692967

Ozcelebi F, et al. Phosphorylation of cholecystokinin receptors expressed on chinese hamster ovary cells. J. Biol. Chem. 271: 3750-3755, 1996. PubMed: 8631990

Lee RJ, Huang L. Folate-targeted, anionic liposome-entrapped polylysine-condensed DNA for tumor cell-specific gene transfer. J. Biol. Chem. 271: 8481-8487, 1996. PubMed: 8626549

Hawes BE, et al. Phosphatidylinositol 3-kinase is an early intermediate in the G beta gamma-mediated mitogen-activated protein kinase signaling pathway. J. Biol. Chem. 271: 12133-12136, 1996. PubMed: 8647803

Parker A, et al. Identification of the extracellular matrix binding sites for insulin-like growth factor-binding protein 5. J. Biol. Chem. 271: 13523-13529, 1996. PubMed: 8662813

Beving DE, et al. Oral immunization with a recombinant cysteine-rich section of the Entamoeba histolytica galactose-inhibitable lectin elicits an intestinal secretory immunoglobulin A response that has in vitro adherence inhibition activity. Infect. Immun. 64: 1473-1476, 1996. PubMed: 8606122

Holtmann MH, et al. Role of receptor phosphorylation in desensitization and internalization of the secretin receptor. J. Biol. Chem. 271: 23566-23571, 1996. PubMed: 8798566

Lugemwa FN, et al. Unusual beta-D-xylosides that prime glycosaminoglycans in animal cells. J. Biol. Chem. 271: 19159-19165, 1996. PubMed: 8702593

Cross References

Nucleotide (GenBank) : U06143 Cricetulus griseus ATCC CCL61 sialidase mRNA, complete cds.

Nucleotide (GenBank) : U20436 Cricetulus griseus furin endoprotease (fur) mRNA, complete cds.

Nucleotide (GenBank) : M87540 Cricetulus longicaudatus sodium channel alpha subunit mRNA, partial cds.

Nucleotide (GenBank) : M87541 Cricetulus longicaudatus sodium channel alpha subunit mRNA, partial cds.

Nucleotide (GenBank) : L13244 Cricetulus griseus CTP:phosphocholine cytidylyltransferase mRNA, complete cds.

Nucleotide (GenBank) : L18986 Cricetulus griseus lysosomal membrane glycoprotein A (lgpA) mRNA, complete cds.

Nucleotide (GenBank) : L19357 Cricetulus griseus lysosomal membrane glycoprotein B (lgpB) mRNA, complete cds.

Nucleotide (GenBank) : AF043911 Cricetulus griseus telomeric repeat binding factor 1 (TRF1) mRNA, partial cds.

Nucleotide (GenBank) : U20435 Cricetulus griseus clone dsi alternatively spliced truncated furin endoprotease (fur) mRNA, complete cds.

Nucleotide (GenBank) : U20434 Cricetulus griseus clone dsii alternatively spliced truncated furin endoprotease (fur) mRNA, complete cds.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Puck TT, et al. Genetics of somatic mammalian cells III. Long-term cultivation of euploid cells from human and animal subjects. J. Exp. Med. 108: 945-956, 1958. PubMed: 13598821

Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc. Natl. Acad. Sci. USA 53: 288-293, 1965. PubMed: 14294058

Kao FT, Puck TT. Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells. Proc. Natl. Acad. Sci. USA 60: 1275-1281, 1968. PubMed: 5244736

Kao FT, Puck TT. Genetics of somatic mammalian cells. IV. Properties of Chinese hamster cell mutants with respect to the requirement for proline. Genetics 55: 513-524, 1967. PubMed: 6068403

Kao FT, et al. Complementation analysis on virus-fused Chinese hamster cells with nutritional markers. Science 164: 312-314, 1969. PubMed: 5813351

Kao F, et al. Genetics of somatic mammalian cells. X. Complementation analysis of glycine-requiring mutants. Proc. Natl. Acad. Sci. USA 64: 1284-1291, 1969. PubMed: 5271752

Mayr GA, Freimuth P. A single locus on human chromosome 21 directs the expression of a receptor for adenovirus type 2 in mouse A9 cells. J. Virol. 71: 412-418, 1997. PubMed: 8985365

Hoppe HC, et al. Identification of phosphatidylinositol mannoside as a mycobacterial adhesin mediating both direct and opsonic binding to nonphagocytic mammalian cells. Infect. Immun. 65: 3896-3905, 1997. PubMed: 9284169

Aragon V, et al. Effect of cytolethal distending toxin on F-actin assembly and cell division in Chinese hamster ovary cells. Infect. Immun. 65: 3774-3780, 1997. PubMed: 9284151

Yang TT, et al. Quantification of gene expression with a secreted alkaline phosphatase reporter system. BioTechniques 23: 1110-1114, 1997. PubMed: 9421645

St. Geme JW, et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830

Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260

Siess DC, et al. Exceptional fusogenicity of chinese hamster ovary cells with murine retrovirus suggests roles for cellular factor(s) and receptor clusters in the membrane fusion process. J. Virol. 70: 3432-439, 1996. PubMed: 8648675

Wang H, et al. Modulation of ecotropic murine retrovirus by N-linked glycosylation of the cell surface receptor/amino acid transporter. J. Virol. 70: 6884-6891, 1996. PubMed: 8794331

Chen Y, et al. Demonstration of binding of dengue virus envelope protein to target cells. J. Virol. 70: 8765-8772, 1996. PubMed: 8971005

Nohturfft A, et al. Recurrent G-to-A substitution in a single codon of SREBP cleavage-activating protein causes sterol resistance in three mutant chinese hamster ovary cell lines. Proc. Natl. Acad. Sci. USA 93: 13709-13714, 1996. PubMed: 8942999

Blanke SR, et al. Fused polycationic peptide mediates delivery on diphtheria toxin A chain to the cytosol in the presence of anthrax protective antigen. Proc. Natl. Acad. Sci. USA 93: 8437-8442, 1996. PubMed: 8710889

Efthimiopoulos S, et al. Cholinergic agonists stimulate secretion of soluble full-length amyloid precursor protein in neuroendocrine cells. Proc. Natl. Acad. Sci. USA 93: 8046-8050, 1996. PubMed: 8755600

Sharma A, et al. Reduction in the level of Gal(alpha 1,3)Gal in transgenic mice and pigs by the expression of an alpha(1,2)fucosyltransferase. Proc. Natl. Acad. Sci. USA 93: 7190-7195, 1996. PubMed: 8692967

Ozcelebi F, et al. Phosphorylation of cholecystokinin receptors expressed on chinese hamster ovary cells. J. Biol. Chem. 271: 3750-3755, 1996. PubMed: 8631990

Lee RJ, Huang L. Folate-targeted, anionic liposome-entrapped polylysine-condensed DNA for tumor cell-specific gene transfer. J. Biol. Chem. 271: 8481-8487, 1996. PubMed: 8626549

Hawes BE, et al. Phosphatidylinositol 3-kinase is an early intermediate in the G beta gamma-mediated mitogen-activated protein kinase signaling pathway. J. Biol. Chem. 271: 12133-12136, 1996. PubMed: 8647803

Parker A, et al. Identification of the extracellular matrix binding sites for insulin-like growth factor-binding protein 5. J. Biol. Chem. 271: 13523-13529, 1996. PubMed: 8662813

Beving DE, et al. Oral immunization with a recombinant cysteine-rich section of the Entamoeba histolytica galactose-inhibitable lectin elicits an intestinal secretory immunoglobulin A response that has in vitro adherence inhibition activity. Infect. Immun. 64: 1473-1476, 1996. PubMed: 8606122

Holtmann MH, et al. Role of receptor phosphorylation in desensitization and internalization of the secretin receptor. J. Biol. Chem. 271: 23566-23571, 1996. PubMed: 8798566

Lugemwa FN, et al. Unusual beta-D-xylosides that prime glycosaminoglycans in animal cells. J. Biol. Chem. 271: 19159-19165, 1996. PubMed: 8702593