IM-9 (ATCC® CCL-159)

Organism: Homo sapiens, human  /  Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transformed  /  Tissue: peripheral blood  /  Disease: multiple myeloma

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Organism Homo sapiens, human
Tissue peripheral blood
Cell Type B lymphoblast; Epstein-Barr virus (EBV) transformed
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 [Cells contain Herpesvirus]
Disease multiple myeloma
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype normal human female; diploid; stable; Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.
Derivation
Although derived from the blood of a patient with multiple myeloma, this line has been shown to be an EBV-transformed B lymphoblastoid cell line.
Clinical Data
Caucasian
female
Antigen Expression
CD11a +; CD19 +; CD20 +; CD38 -; CD49e +
Receptor Expression
growth hormone receptor
insulin receptor
calcitonin receptor
Genes Expressed
immunoglobulin
Cellular Products
immunoglobulin
Comments

The cells are EBNA positive. 

ATCC confirmed this cell line is positive for the presence of Epstein-Barr (EBV) viral DNA sequences via PCR.

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL. Maintain cell density between 1x 105 and 1 x 106 viable cells/mL. Do not allow the cell density to exceed 2 x 106 cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10,11
D13S317: 9,11
D16S539: 9,13
D5S818: 13
D7S820: 11,12
THO1: 6,9.3
TPOX: 11
vWA: 14,17
Isoenzymes
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 2
PGM1, 1-2
PGM3, 0
Isotype IgG kappa , IgG; kappa light chain Refvan Boxel JA, Buell DN. IgD on cell membranes of human lymphoid cell lines with multiple immunoglobulin classes. Nature 251: 443-444, 1974. PubMed: 4608784
Name of Depositor DN Buell
Year of Origin 1967
References

Fahey JL, et al. Proliferation and differentiation of lymphoid cells: studies with human lymphoid cell lines and immunoglobulin synthesis. Ann. N.Y. Acad. Sci. 190: 221-234, 1971. PubMed: 5290016

Lesniak MA, et al. Binding of 125I-human growth hormone to specific receptors in human cultured lymphocytes. Characterization of the interaction and a sensitive radioreceptor assay. J. Biol. Chem. 249: 1661-1667, 1974. PubMed: 4361817

Van Obberghen E, et al. Cell surface receptors for insulin and human growth hormone. Effect of microtubule and microfilament modifiers. J. Biol. Chem. 251: 6844-6851, 1976. PubMed: 185222

Gavin JR, et al. Insulin-dependent regulation of insulin receptor concentrations: a direct demonstration in cell culture. Proc. Natl. Acad. Sci. USA 71: 84-88, 1974. PubMed: 4359334

van Boxel JA, Buell DN. IgD on cell membranes of human lymphoid cell lines with multiple immunoglobulin classes. Nature 251: 443-444, 1974. PubMed: 4608784

Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375

Drexler H, et al. False human hematopoietic cell lines: cross-contaminations and misinterpretations. Leukemia 13: 1601-1607, 1999. PubMed: 10516762

van Boxel JA, Buell DN. IgD on cell membranes of human lymphoid cell lines with multiple immunoglobulin classes. Nature 251: 443-444, 1974. PubMed: 4608784

Cross References

Nucleotide (GenBank) : X81121 H.sapiens mRNA for central cannabinoid receptor, short isoform.

Nucleotide (GenBank) : X84369 H.sapiens RNA for heavy chain variable and constant regions (IM9-VH).

Nucleotide (GenBank) : U07989 Human Burkitt's lymphoma immunoglobulin kappa light chain mRNA, partial cds.

Nucleotide (GenBank) : U07985 Human myeloma immunoglobulin heavy chain Fd region V-D-J-CH1 mRNA, partial cds.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Fahey JL, et al. Proliferation and differentiation of lymphoid cells: studies with human lymphoid cell lines and immunoglobulin synthesis. Ann. N.Y. Acad. Sci. 190: 221-234, 1971. PubMed: 5290016

Lesniak MA, et al. Binding of 125I-human growth hormone to specific receptors in human cultured lymphocytes. Characterization of the interaction and a sensitive radioreceptor assay. J. Biol. Chem. 249: 1661-1667, 1974. PubMed: 4361817

Van Obberghen E, et al. Cell surface receptors for insulin and human growth hormone. Effect of microtubule and microfilament modifiers. J. Biol. Chem. 251: 6844-6851, 1976. PubMed: 185222

Gavin JR, et al. Insulin-dependent regulation of insulin receptor concentrations: a direct demonstration in cell culture. Proc. Natl. Acad. Sci. USA 71: 84-88, 1974. PubMed: 4359334

van Boxel JA, Buell DN. IgD on cell membranes of human lymphoid cell lines with multiple immunoglobulin classes. Nature 251: 443-444, 1974. PubMed: 4608784

Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375

Drexler H, et al. False human hematopoietic cell lines: cross-contaminations and misinterpretations. Leukemia 13: 1601-1607, 1999. PubMed: 10516762

van Boxel JA, Buell DN. IgD on cell membranes of human lymphoid cell lines with multiple immunoglobulin classes. Nature 251: 443-444, 1974. PubMed: 4608784