C6 (ATCC® CCL-107)

Organism: Rattus norvegicus, rat  /  Cell Type: glial cell  /  Tissue: brain  /  Disease: glioma

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Organism Rattus norvegicus, rat
Tissue brain
Cell Type glial cell
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease glioma
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype Stemline number is diploid. Karyotype is stable within the stemline number and is that of a normal male. Three cells with breaks; one with a secondary constriction, one with a dicentric, one with a rearrangement and four with terminal or centromere associations. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.
Derivation
The glial cell strain, C6, was cloned from a rat glial tumor induced by N-nitrosomethylurea by Benda et al. after a series of alternate culture and animal passages.
Receptor Expression
glucocorticoid receptor
Genes Expressed
S-100 protein; produce glyceryl phosphate dehydrogenase in response to glucocorticoids; somatotrophin
Cellular Products
S-100 protein; produce glyceryl phosphate dehydrogenase in response to glucocorticoids; somatotrophin
Virus Susceptibility Vaccinia virus
Vesicular stomatitis, Orsay (Indiana)
Vesicular stomatitis, Glasgow (Indiana)
Herpes simplex virus
Virus Resistance
poliovirus 3
Comments
S-100 production increases ten fold as cells grow from low density to confluency.
Complete Growth Medium The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Name of Depositor G Sato
References

Benda P, et al. Differentiated rat glial cell strain in tissue culture. Science 161: 370-371, 1968. PubMed: 4873531

Lightbody JJ, et al. Establishment of differentiated clonal strains of glial brain cells in culture. Fed. Proc. 27: 720, 1968.

Chen Y, et al. Demonstration of binding of dengue virus envelope protein to target cells. J. Virol. 70: 8765-8772, 1996. PubMed: 8971005

Cross References

Nucleotide (GenBank) : X89603 R.norvegicus mRNA for metallothionein-III.

Nucleotide (GenBank) : E01331 Rat glia-derived neurite-promoting factor gene.

Nucleotide (GenBank) : AF069306 Rattus norvegicus transaldolase mRNA, complete cds.

Nucleotide (GenBank) : AF202115 Rattus norvegicus GPI-anchored ceruloplasmin mRNA, complete cds.

Nucleotide (GenBank) : X60002 Rat delta CREB mRNA for cAMP-responsive element (CRE) binding protein.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Benda P, et al. Differentiated rat glial cell strain in tissue culture. Science 161: 370-371, 1968. PubMed: 4873531

Lightbody JJ, et al. Establishment of differentiated clonal strains of glial brain cells in culture. Fed. Proc. 27: 720, 1968.

Chen Y, et al. Demonstration of binding of dengue virus envelope protein to target cells. J. Virol. 70: 8765-8772, 1996. PubMed: 8971005