VS1742 Idaho - 1 (ATCC® 87715)

Organism: Cryptosporidium sp.  /  Clone Type: Library  /  Depositors: SJ Upton, NV Khramtsov

Permits and Restrictions

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Designations VS1742 Idaho - 1
Species Cryptosporidium sp.
Depositors SJ Upton, NV Khramtsov
Origin
sporozoites/oocysts
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II SK+
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1, f1
Cross references:
Vector
Tissue: sporozoites/oocysts
DNA: genomic
Digest: Complete
Insert size range: 0.1 - 2.3
Coverage: Clones for "complete" coverage: 34000.0
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: frozen
Comments
The VS1742 isolate was collected from the feces of a 3 1/2 year old heifer near Caldwell, Idaho. Viable oocysts (average: 8.38 x 6.19 um) were purified from feces on CsCl gradient then surface sterilized with 10% Clorox prior to nucleic acid extraction.
References

Nikolai V Khramtsov, personal communication

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