pWE1 (ATCC® 87671)

Applications: expression vector  /  Depositors: M Cockett

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Designations pWE1
Depositors M Cockett
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 6.8590002059936520
DESCRIPTION OF VECTOR:
Intact vector size: 6.859
Type of vector: phagemid
Cloning sites: HindIII BspMI PstI XbaI BamHI EcoRI
Polylinker sites: HindIII BspMI PstI SalI AccI XbaI BamHI AvaI XmaI SmaI EcoRI
ApaI
Host range: mammalian cells; Escherichia coli
Features (with orientation and position when available):
MCS: HindIII...ApaI, ->, 1-63
promoter for in vitro transcription: SP6, <-, 68-84
terminator: SV40 polyadenylation
enhancer: SV40, 1099-1242
replicon: SV40, 1266-1401
marker(s): mycophenolic acid resistance (GPT), ->, 1614-2072
replicon: pMB1, <-, 3636-3636
marker(s): ampR, <-, 4394-5254
replicon: f1 ori, ->, 5385-5841
promoter for expression: CMV, ->, 6203-6790
promoter for in vitro transcription: T7, ->, 6834-6853
Vector: pWE1 (phagemid)
Promoters: Promoter for in vitro transcription SP6
Marker(s):ampR,GPT
Construct size (kb): 6.859000205993652
Features: marker(s): ampR
marker(s): mycophenolic acid resistance (GPT)
promoter for expression: CMV
promoter for in vitro transcription: SP6
promoter for in vitro transcription: T7
replicon: SV40
replicon: f1 ori
replicon: pMB1
MCS: HindIII...ApaI
terminator: SV40 polyadenylation
enhancer: SV40
Applications
expression vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.8; SalI--3.7, 3.3.
Having identical cloning sites also facilitates shuttling cDNAs from one vector to another.
One of a series of vectors (ATCC 87671 - 87674) that allows simultaneous, high-level expression of multiple cDNA sequences in mammalian cells. A kit of all four vectors is also available (ATCC 87678).
Each cDNA inserted into one of this series of vectors is regulated by an identical transcription unit, but each vector permits different selection to be applied to different subunits.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Cockett MI, et al. Simultaneous expression of multi-subunit proteins in mammalian cells using a convenient set of mammalian cell expression vectors. BioTechniques 23: 402-407, 1997. PubMed: 9298206

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component of:ATCC 87678
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Shipping Information Distributed: freeze-dried
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