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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 7.6250000000000000 Vector: pCM184 (plasmid) Promoters: Promoter CMV Marker(s):TRP1,ampR Construct size (kb): 7.625 Features: marker(s): TRP1
marker(s): ampR
operator: tetO - seven repeats
other: tTA transcriptional activator
promoter: CMV
promoter: CYC1
replicon: ARS1
replicon: pMB1
MCS: BamHI...PstI
terminator: ADH1
terminator: CYC1
centromere: CEN4
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Applications
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YC-type (centromeric) shuttle vector expression vector
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Comments
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Restriction digests of the clone give the following sizes (kb): BamHI--7.5; PstI--7.5; BamHI/XhoI--6.6, 0.76. Yeast centromeric expression vector driven by the tetO-CYC1 promoter. One of 7 tetracycline-regulated expression vectors ( ATCC 87656 - 87662) differing in the level of expression, mode of replication and selectable marker. |
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References
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Gari E, et al. A set of vectors with a tetracycline-regulatable promoter system for modulated gene expression in Saccharomyces cerevisiae. Yeast 13: 837-848, 1997. PubMed: 9234672
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