YEpFAT6 (ATCC® 87644)

Applications: M9 + leucine + tryptophan medium recommended for DNA purification.  /  Depositors: K Runge

Permits and Restrictions

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Designations YEpFAT6
Depositors K Runge
Biosafety Level 1
Host
Distribution host: Escherichia coli MC1066
Vector Information
Size (kb): 10.6840000152587900
DESCRIPTION OF VECTOR:
Intact vector size: 10.684
Type of vector: plasmid
Cloning sites: SacI SmaI SalI
Polylinker sites: EcoRI SacI AvaI KpnI SmaI BamHI XbaI SalI PstI HindIII
Other unique sites: NotI
Host range: Schizosaccharomyces pombe; Saccharomyces cerevisiae;
Escherichia coli
Features (with orientation and position when available):
promoter for in vitro transcription: T7, ->, 9382-9401
MCS: EcoRI...HindIII, ->, 10635-2
marker(s): GART, <-, 2-2091
replicon: 2 micron/LEU2-d, 2086-4780
marker(s): LEU2-d, ->, 3443-4537
marker(s): URA4, <-, 4928-5722
promoter for in vitro transcription: T3, <-, 6261-6280
promoter: lac, <-, 6350-6378
replicon: pMB1, 6704-6704
marker(s): ampR, <-, 7462-8322
Vector: YEpFAT6 (plasmid)
Promoters: Promoter for in vitro transcription T7
Marker(s):ampR,LEU2-d,ura4+
Construct size (kb): 10.68400001525879
Features: marker(s): GART
marker(s): LEU2-d
marker(s): URA4
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: 2 micron/LEU2-d
replicon: pMB1
MCS: EcoRI...HindIII
Applications
M9 + leucine + tryptophan medium recommended for DNA purification.
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--10.0. 1.0; SmaI--11.0; HindIII--6.55, 4.8.
M9 + leucine + tryptophan medium recommended for DNA purification.
One of 9 YE-type shuttle vectors (ATCC 87541, 87554, 87560 an d 87640 - 87645) that have the leu2-d gene, which is correlated with high copy number in S. cerevisiae, when grown in medium lacking leucine.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Runge KW, Zakian VA. Introduction of extra telomeric DNA sequences into Saccharomyces cerevisiae results in telomere elongation. Mol. Cell. Biol. 9: 1488-1497, 1989. PubMed: 2657397

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
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