YEpFAT5 (ATCC® 87643)

Applications: M9 + leucine + tryptophan medium recommended for DNA purification.Selection for URA3 expression produces a plasmid copy number of 20-40; growth on YC minus leucine produces a copy number of 100-200.  /  Depositors: K Runge

Permits and Restrictions

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Designations YEpFAT5
Depositors K Runge
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli MC1066
Vector Information
Size (kb): 7.1700000762939450
DESCRIPTION OF VECTOR:
Intact vector size: 7.170
Type of vector: plasmid
Cloning sites: NotI SacI SmaI BamHI XbaI
Polylinker sites: NotI EcoRI SacI AvaI KpnI SmaI BamHI XbaI
Other unique sites: HindIII
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
promoter for in vitro transcription: T7, ->, 860-879
MCS: NotI...XbaI, ->, 908-942
replicon: 2 micron/LEU2-d, 941-3794
marker(s): LEU2-d, ->, 2298-3392
marker(s): URA3, <-, 3874-4676
restriction site: HindIII, 4899-4899
promoter for in vitro transcription: T3, <-, 4909-4928
promoter: lac, <-, 4998-5026
replicon: pMB1, 5352-5352
marker(s): ampR, <-, 6110-6970
Vector: YEpFAT5 (plasmid)
Promoters: Promoter for in vitro transcription T7
Marker(s):URA3,ampR,LEU2-d
Construct size (kb): 7.170000076293945
Features: marker(s): LEU2-d
marker(s): URA3
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: 2 micron/LEU2-d
replicon: pMB1
MCS: NotI...XbaI
restriction site: HindIII
Applications
M9 + leucine + tryptophan medium recommended for DNA purification.
Selection for URA3 expression produces a plasmid copy number of 20-40; growth on YC minus leucine produces a copy number of 100-200.
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--7.0; SmaI--7.0; EcoRI--4.4, 2.0, 0.8.
M9 + leucine + tryptophan medium recommended for DNA purification.
Selection for URA3 expression produces a plasmid copy number of 20-40; growth on YC minus leucine produces a copy number of 100-200.
One of 9 YE-type shuttle vectors (ATCC 87541, 87554, 87560 an d 87640 - 87645) that have the leu2-d gene, which is correlated with high copy number in S. cerevisiae, when grown in medium lacking leucine.
Media M9 + tryptophan + leucine
Growth Conditions
Temperature: 37.0°C
References

Runge KW, Zakian VA. Introduction of extra telomeric DNA sequences into Saccharomyces cerevisiae results in telomere elongation. Mol. Cell. Biol. 9: 1488-1497, 1989. PubMed: 2657397

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 87643