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YEpFAT5 (ATCC^® 87643^™)

Applications: M9 + leucine + tryptophan medium recommended for DNA purification.Selection for URA3 expression produces a plasmid copy number of 20-40; growth on YC minus leucine produces a copy number of 100-200. / Depositors: K Runge

YEpFAT5 ATCC^® 87643^™

freeze-dried

For-Profit: $290.00 Non-Profit: $290.00

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Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Designations	YEpFAT5
Depositors	K Runge
Biosafety Level	1
Vector Information	Size (kb): 7.1700000762939450 DESCRIPTION OF VECTOR: Intact vector size: 7.170 Type of vector: plasmid Cloning sites: NotI SacI SmaI BamHI XbaI Polylinker sites: NotI EcoRI SacI AvaI KpnI SmaI BamHI XbaI Other unique sites: HindIII Host range: Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): promoter for in vitro transcription: T7, ->, 860-879 MCS: NotI...XbaI, ->, 908-942 replicon: 2 micron/LEU2-d, 941-3794 marker(s): LEU2-d, ->, 2298-3392 marker(s): URA3, <-, 3874-4676 restriction site: HindIII, 4899-4899 promoter for in vitro transcription: T3, <-, 4909-4928 promoter: lac, <-, 4998-5026 replicon: pMB1, 5352-5352 marker(s): ampR, <-, 6110-6970 Vector: YEpFAT5 (plasmid) Promoters: Promoter for in vitro transcription T7 Marker(s):URA3,ampR,LEU2-d Construct size (kb): 7.170000076293945 Features: marker(s): LEU2-d marker(s): URA3 marker(s): ampR promoter: lac promoter for in vitro transcription: T3 promoter for in vitro transcription: T7 replicon: 2 micron/LEU2-d replicon: pMB1 MCS: NotI...XbaI restriction site: HindIII
Applications	M9 + leucine + tryptophan medium recommended for DNA purification. Selection for URA3 expression produces a plasmid copy number of 20-40; growth on YC minus leucine produces a copy number of 100-200.
Comments	Restriction digests of the clone give the following sizes (kb): BamHI--7.0; SmaI--7.0; EcoRI--4.4, 2.0, 0.8. M9 + leucine + tryptophan medium recommended for DNA purification. Selection for URA3 expression produces a plasmid copy number of 20-40; growth on YC minus leucine produces a copy number of 100-200. One of 9 YE-type shuttle vectors (ATCC 87541, 87554, 87560 an d 87640 - 87645) that have the leu2-d gene, which is correlated with high copy number in S. cerevisiae, when grown in medium lacking leucine.
Media	M9 + tryptophan + leucine
Growth Conditions	Temperature: 37.0°C
References	Runge KW, Zakian VA. Introduction of extra telomeric DNA sequences into Saccharomyces cerevisiae results in telomere elongation. Mol. Cell. Biol. 9: 1488-1497, 1989. PubMed: 2657397
Shipped	FD
Shipping Information	Distributed: freeze-dried

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Product Sheet	Product Sheet
Attachments	Vector map for 87643