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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 5.6030001640319820 DESCRIPTION OF VECTOR:
Intact vector size: 5.603
Type of vector: plasmid
Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Other unique sites: EcoRV NdeI AatII NarI SpeI NaeI NheI BglII ClaI NsiI StuI
Construction: pUC19
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
promoter: lac, ->, 143-171
insert detection: lacZ', ->, 216-500
MCS: HindIII...EcoRI, ->, 234-285
centromere: CEN4, 504-1480
replicon: ARS1, 1481-2225
marker(s): URA3, <-, 2563-3365
marker(s): ampR, ->, 3802-4662
replicon: pMB1, 5420-5420 Vector: YCplac33 (plasmid) Promoters: Promoter lac Construction: pUC19 Marker(s):URA3,ampR Construct size (kb): 5.603000164031982 Features: insert detection: lacZ'
marker(s): URA3
marker(s): ampR
promoter: lac
replicon: ARS1
replicon: pMB1
MCS: HindIII...EcoRI
centromere: CEN4
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Applications
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YC-type (centromeric) shuttle vector vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
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Comments
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Restriction digests of the clone give the following sizes (kb): AvaI--3.7, 1.9; ClaI--5.7; PstI--5.6. One of 9 shuttle vectors ( ATCC 87585 - 87593) allowing lacZ detection of cloned inserts and containing the pUC19 MCS with all 10 cloning sites unique. Vectors differ in mode of replication (YE, YC or YI-type) and yeast marker (LEU2, URA3 or TRP1). The PstI restriction site from the URA3 gene of S. cerevisiae were removed by using oligo-directed mutagenesis.
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References
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Gietz RD, Sugino A. New yeast Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74: 527-534, 1988. PubMed: 3073106
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Related Products
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component of:ATCC 87638
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Shipping Information
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Distributed: freeze-dried
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