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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 10.3059997558593800 Vector: pRS319a (phagemid) Promoters: Promoter for in vitro transcription T7 Construction: pRS315 Marker(s):LEU2,ampR,CAN1 Construct size (kb): 10.30599975585938 Features: marker(s): CAN1
marker(s): LEU2
marker(s): ampR
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: ARS4
replicon: f1
replicon: pMB1
MCS: SacI...KpnI
centromere: CEN6
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Applications
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YC-type (centromeric) shuttle vector
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Comments
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Restriction digests of the clone give the following sizes (kb): EcoRI--4.1, 2.8, 2.6, 1.1; HindIII--6.6, 2.8, 1.4; PstI--9.5, 1.0. pRS319a differs from pRS319 in that pRS319 has a truncated CAN1 gene and pRS319a has a full length CAN1 gene. A plasmid shuffling vector. YC-type centromere vector permitting visual detection of recombinants and production of ssDNA in E. coli. Contains CAN1 marker (full length CAN1 gene) for negative selection. pRS319a was constructed by inserting a 4.161 kb PvuII-Sma CAN1 fragment from M3093 (CAN1 in pBluescript II) into the HpaI site (position 2175) of pRS315 ( ATCC 77144). The order of the major features in this plasmid is: LEU2 - CAN1 - f1 ori - T7 promoter - lacZ'/MCS - T3 promoter - pMB1 ori - bla - CEN6 - ARSH4.
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References
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Sikorski RS, Boeke JD. In vitro mutagenesis and plasmid shuffling: from cloned gene to mutant gene. Methods Enzymol. 194: 302-318, 1991. PubMed: 2005795
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