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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 4.788 Vector: pRS414 (phagemid) Promoters: Promoter for in vitro transcription T7 Construction: pBluescriptII KS+ Marker(s):TRP1,ampR Construct size (kb): 4.788 Features: insert detection: lacZ'
marker(s): TRP1
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: ARSH4
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
centromere: CEN6
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Applications
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YC-type (centromeric) shuttle vector
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Comments
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Restriction digests of the clone give the following sizes (kb): PvuII--4.35, 0.45; BglI--3.1, 1.7; HindIII--3.85, 0.95. A YC-type shuttle vector with TRP1 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter system for visual detection of recombinants, promoters for transcription in vitro, and an extensive polylinker flanked by BssHII sites.
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References
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Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436
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Shipping Information
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Freeze dried phagemid in E. coli
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