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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 4.2740001678466800 Vector: pRS404 (phagemid) Promoters: Promoter T7 Construction: pBluescript II KS+ Marker(s):TRP1,ampR Construct size (kb): 4.27400016784668 Features: insert detection: lacZ'
marker(s): TRP1
marker(s): ampR
promoter: T3
promoter: T7
promoter: lac
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
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Applications
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YI-type (integrating) shuttle vector
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Comments
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Restriction digests of the clone give the following sizes (kb): HindIII--3.35, 0.95; BglI--2.6, 1.7; PvuII--3.85, 0.45. A YI-type shuttle vector with TRP1 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter system for visual detection of recombinants, promoters for transcription in vitro, and an extensive polylinker flanked by BssHII sites.
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References
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Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436
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