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Vector Information
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Size (kb): 4.456 Vector: pRS403 (phagemid) Promoters: Promoter for in vitro transcription T7 Construction: pBluescriptII KS+ Marker(s):ampR,HIS3 Construct size (kb): 4.456 Features: insert detection: lacZ'
marker(s): HIS3
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
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Applications
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YI-type (integrating) shuttle vector
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Comments
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Restriction digests of the clone give the following sizes (kb): PvuII--4.0, 0.45; BglI--1.7, 1.7, 1.05; PstI--3.5, 0.95. A YI-type shuttle vector with HIS3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter system for visual detection of recombinants, promoters for transcription in vitro, and an extensive polylinker flanked by BssHII sites.
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References
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Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436
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