pRS412 (ATCC® 87478)

Applications: YC-type (centromeric) shuttle vectorvector permitting visual detection of recombinants phosphoribosylaminoimidazole succinocarboxamide synthetase SAICAR synthetase, phosphoribosylaminoimidazole carboxylase  /  Depositors: JD Boeke

Permits and Restrictions

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Designations pRS412
Depositors JD Boeke
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 6.042
DESCRIPTION OF VECTOR:
Intact vector size: 6.042
Type of vector: phagemid
Cloning sites: SacI SacII EagI NotI SpeI BamHI SmaI PstI EcoRI ClaI SalI
XhoI ApaI KpnI
Polylinker sites: SacI BstXI SacII EagI NotI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII ClaI SalI XhoI ApaI KpnI
Construction: pRS402, pRS411
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): ADE2, ->, 575-2290
replicon: f1, <-, 2495-2951
promoter for in vitro transcription: T7, ->, 3121-3140
MCS: KpnI...SacI, ->, 3148-3250
promoter for in vitro transcription: T3, <-, 3267-3286
insert detection: lacZ', <-, 2952-3311
promoter: lac, <-, 3356-3384
replicon: pMB1, 3710-3710
marker(s): ampR, <-, 4468-5328
centromere: CEN6, 5470-5586
replicon: ARS4, 5587-5988
Vector: pRS412 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pRS402, pRS411
Marker(s):ampR,ADE2
Construct size (kb): 6.042
Features: insert detection: lacZ'
marker(s): ADE2
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: ARS4
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
centromere: CEN6
Applications
YC-type (centromeric) shuttle vector
vector permitting visual detection of recombinants phosphoribosylaminoimidazole succinocarboxamide synthetase SAICAR synthetase, phosphoribosylaminoimidazole carboxylase
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.0; BglII--2.2, 3.8; EcoRI--6.0.
ade2 phenotype produces red colonies when grown on adenine containing media.
This vector is useful for gene knockout experiments in hosts with a non-revertable ade2 auxotrophic marker gene mutation.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801

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Shipped frozen
Shipping Information Distributed: freeze-dried
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