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pRS411 (ATCC^® 87474^™)

Applications: YC-type (centromeric) shuttle vectorvector permitting visual detection of recombinants O-acetylhomoserine sulfhydrylase / Depositors: JD Boeke

pRS411 ATCC^® 87474^™

frozen

For-Profit: $290.00 Non-Profit: $290.00

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Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Designations	pRS411
Depositors	JD Boeke
Biosafety Level	1
Vector Information	Size (kb): 5.407 DESCRIPTION OF VECTOR: Intact vector size: 5.407 Type of vector: phagemid Cloning sites: SacI BstXI SacII EagI NotI SpeI BamHI SmaI PstI ClaI SalI XhoI ApaI KpnI Polylinker sites: SacI BstXI SacII EagI NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV ClaI SalI XhoI ApaI KpnI Construction: pRS401, pRS84 Host range: Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): marker(s): MET15, ->, 421-1270 replicon: f1, <-, 1860-2316 promoter for in vitro transcription: T7, ->, 2486-2505 MCS: KpnI...SacI, ->, 2513-2615 promoter for in vitro transcription: T3, <-, 2632-2651 coding sequence: lacZ', <-, 2317-2676 promoter: lac, <-, 2721-2749 replicon: pMB1, 3075-3075 marker(s): ampR, <-, 3833-4693 centromere: CEN6, 4835-4951 replicon: ARS4, 4952-5353 Vector: pRS411 (phagemid) Promoters: Promoter for in vitro transcription T7 Construction: pRS401, pRS84 Marker(s):ampR,MET15 Construct size (kb): 5.407 Features: marker(s): MET15 marker(s): ampR promoter: lac promoter for in vitro transcription: T3 promoter for in vitro transcription: T7 replicon: ARS4 replicon: f1 replicon: pMB1 MCS: KpnI...SacI centromere: CEN6 coding sequence: lacZ'
Applications	YC-type (centromeric) shuttle vector vector permitting visual detection of recombinants O-acetylhomoserine sulfhydrylase
Comments	Restriction digests of the clone give the following sizes (kb): BamHI--5.4; EcoRI--3.7, 1.7; XbaI--3.4, 2.0. met15 phenotype produces brown colonies when grown on Pb containing media. MET15, MET17 and MET25 are synonymous. This vector is useful for gene knockout experiments in hosts with a non-revertable met15 auxotrophic marker gene mutation.
Media	ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions	Temperature: 37.0°C
References	Cost GJ, Boeke JD. A useful colony colour phenotype associated with the yeast selectable/counter-selectable marker MET15. Yeast 12: 939-941, 1996. PubMed: 8873447 Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801
Related Products	component of:ATCC 87476 component of:ATCC 87529
Shipped	FZ
Shipping Information	Distributed: freeze-dried

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Product Sheet	Product Sheet
Attachments	Vector map for 87474