pUC19Sce [pUCSce] (ATCC® 87437)

Applications: vector permitting visual detection of recombinants  /  Depositors: HP Schweizer

Designations pUC19Sce [pUCSce]
Depositors HP Schweizer
Biosafety Level 1
Vector Information
Size (kb): 2.6860001087188720
Vector: pUC19Sce (plasmid)
Construction: pUC19
Marker(s):ampR
Construct size (kb): 2.686000108718872
Features: marker(s): ampR
replicon: pMB1
restriction site: I-SceI
restriction site: SmaI
coding sequence: lacZalpha
Applications
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): SmaI--2.7; AvaI--2.7; BglI--1.6, 1.1.
Useful for rescuing cloned bacterial inserts by digestion with I-SceI, an enzyme recognizing a rare 18 bp sequence which has yet to be found in bacterial DNA.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Schweizer HP, Hoang TT. An improved system for gene replacement and xylE fusion analysis in Pseudomonas aeruginosa. Gene 158: 15-22, 1995. PubMed: 7789804

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet
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