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pBAD28 (ATCC^® 87400^™)

Applications: expression vectorproduces protein arabinose regulatorvector containing primer sites useful for sequencingvector with low copy number / Depositors: L Guzman, J Beckwith

pBAD28 ATCC^® 87400^™

frozen

For-Profit: $190.00 Non-Profit: $190.00

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Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Designations	pBAD28
Depositors	L Guzman, J Beckwith
Biosafety Level	1
Vector Information	Vector Information: Vector type: phagemid Vector size (kb): 5.801 Markers: ampR, cmlR, araC Polylinker sites: NheI, EcoRI, SacI, KpnI, SmaI, BamHI, XbaI, SalI, AccI, PstI, SphI, HindIII Construction: pBAD18, pACYC184 Features: marker(s): ampR marker(s): cmlR operator: I2 + I1 operator: O1 operator: O2 other: CAP site promoter: araC promoter: bla (ampR) promoter for expression: arabinose BAD regulator: araC replicon: M13 replicon: p15A MCS: NheI...HindIII transcription terminator: rrnB T1 + T2
Applications	expression vector produces protein arabinose regulator vector containing primer sites useful for sequencing vector with low copy number
Comments	Restriction digests of the clone give the following sizes (kb): AvaI--3.1, 1.9, 1.3; BamHI--6.1; PstI--5.0;1.2. Cultures should be grown in minimal media for more reproducible induction of expression. Expression is induced in glycerol-containing media by addition of arabinose. Expression is repressed by addition of glucose or other catabolites. One of several tightly controlled expression vectors (ATCC 87393-87402) regulated by the arabinose operon. The vectors differ in replicon, antibiotic resistance marker, multiple cloning site and mechanism of initiation of translation. The following primers can be used for sequencing of cloned inserts: 5' primer (27 - 8 bp upstream of the NheI site) 5'-CTGTTTCTCCATACCCGTT-3'; and one of two 3' primers: 3' primer 1 (2 - 19 bp downstream of the HindIII site) 5'-CTCATCCGCCAAAACAG-3'; 3' primer 2 (17 - 33 bp downstream of the HindIII site) 5'-GGCTGAAAATCTTCTCT-3'. Plasmid copy number is low due to the p15A replicon. This vector can be used when reduced gene expression is desirable. Plasmid is compatible with pBR-derived plasmids and may be used for coexpression of cloned inserts.
Media	ATCC^® Medium 1949: LB medium (ATCC medium 1065) with 50 mcg/ml chloramphenicol and 100 mcg/ml ampicillin
Growth Conditions	Temperature: 37°C
References	Guzman LM, et al. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J. Bacteriol. 177: 4121-4130, 1995. PubMed: 7608087
Shipped	FZ
Shipping Information	Frozen glycerol stock of E. coli DH5-alpha; containing the plasmid.

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Product Sheet	Product Sheet
Attachments	Vector Map Product Information Sheet