p414 CYC1 (ATCC® 87380)

Applications: YX-type (expression) shuttle vectorvector with low copy number  /  Depositors: M Funk

Permits and Restrictions

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Designations p414 CYC1
Depositors M Funk
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 5.3010001182556150
DESCRIPTION OF VECTOR:
Intact vector size: 5.301
Type of vector: plasmid
Cloning sites: SalI ClaI EcoRI PstI SmaI BamHI SpeI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS414
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
replicon: CEN6/ARSH4, ->
marker(s): TRP1, ->
terminator: CYC1, ->
promoter for expression: CYC1, <-
marker(s): ampR, <-
Vector: p414 CYC1 (plasmid)
Promoters: Promoter for expression CYC1
Construction: pRS414
Marker(s):TRP1,ampR
Construct size (kb): 5.301000118255615
Features: marker(s): TRP1
marker(s): ampR
promoter for expression: CYC1
replicon: CEN6/ARSH4
terminator: CYC1
Applications
YX-type (expression) shuttle vector
vector with low copy number
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.6; EcoRI--5.6; SacI/XbaI--3.4, 1.7.
Contains a truncated version of the CYC1 promoter that very weak and no longer inducible because of a deletion of most of the UAS2 sequence.
Low copy number shuttle expression vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

Related Products
component of:ATCC 87669
Shipped frozen
Shipping Information Distributed: freeze-dried
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