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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 6.527 DESCRIPTION OF VECTOR:
Intact vector size: 6.527
Type of vector: plasmid
Cloning sites: XhoI SalI ClaI EcoRI PstI SmaI BamHI SpeI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS424
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
replicon: 2 micron, ->
marker(s): TRP1, ->
terminator: CYC1, ->
promoter for expression: GPD, <-
marker(s): ampR, <- Vector: p424 GPD (plasmid) Promoters: Promoter for expression GPD Construction: pRS424 Marker(s):TRP1, ampR Construct size (kb): 6.527 Features: Marker(s): TRP1, ampR Promoter for expression: GPD Replicon: 2 micron Terminator: CYC1
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Applications
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YX-type (expression) shuttle vector
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Comments
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Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 1.7, 1.0, 0.68; EcoRI--6.5; BamHI--6.5. High copy number shuttle expression vector.
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References
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Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504
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Shipping Information
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Distributed: freeze-dried
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