p414 GALS (ATCC® 87344)

Applications: YX-type (expression) shuttle vectorvector with low copy number  /  Depositors: M Funk

Permits and Restrictions

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Designations p414 GALS
Depositors M Funk
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 5.4289999008178710
Intact vector size: 5.429
Type of vector: plasmid
Cloning sites: SpeI BamHI SmaI PstI EcoRI ClaI SalI XhoI
Polylinker sites: XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI
Construction: pRS414, GALS promoter
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): TRP1, ->, 200-1213
terminator: CYC1, ->, 1895-2155
MCS: XhoI...SpeI, ->, 2155-2212
promoter for expression: GALS, <-, 2219-2634
marker(s): ampR, <-, 3881-4811
centromere: CEN6/ARSH4, ->, 4915-5429
Vector: p414 GALS (plasmid)
Promoters: Promoter for expression GALS
Construction: pRS414, GALS promoter
Construct size (kb): 5.428999900817871
Features: marker(s): TRP1
marker(s): ampR
promoter for expression: GALS
MCS: XhoI...SpeI
terminator: CYC1
centromere: CEN6/ARSH4
YX-type (expression) shuttle vector
vector with low copy number
Restriction digests of the clone give the following sizes (kb): BamHI--5.4; EcoRI--5.4; SacI/XbaI--3.2, 1.8, 0.41.
The galactokinase (GALS) promoter is a deletion variant of GAL1, which lacks one and a half of the 3 UAS elements required for full induction of the promoter by galactose.
The promoter is tightly repressed by glucose and is induced at a moderate level by galactose.
Low copy number shuttle expression vector.
One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736

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component of:ATCC 87670
Shipped freeze-dried
Shipping Information Distributed: freeze-dried
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Attachments Vector map for 87344