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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 5.5539999008178710 DESCRIPTION OF VECTOR:
Intact vector size: 5.554
Type of vector: plasmid
Cloning sites: XbaI SpeI BamHI SmaI EcoRI HindIII ClaI SalI XhoI
Polylinker sites: XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI
Construction: pRS416, GALL promoter
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->, 200-1323
terminator: CYC1, ->, 2005-2265
MCS: XhoI...XbaI, ->, 2265-2328
promoter for expression: GALL, <-, 2329-2759
marker(s): ampR, <-, 4006-4936
centromere: CEN6/ARSH4, ->, 5040-5554 Vector: p416 GALL (plasmid) Promoters: Promoter for expression GALL Construction: pRS416, GALL promoter Marker(s):URA3,ampR Construct size (kb): 5.553999900817871 Features: marker(s): URA3
marker(s): ampR
promoter for expression: GALL
MCS: XhoI...XbaI
terminator: CYC1
centromere: CEN6/ARSH4
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Applications
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YX-type (expression) shuttle vector vector with low copy number
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Comments
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Restriction digests of the clone give the following sizes (kb): BamHI--5.6; EcoRI--5.6; SacI/XbaI--5.1, 0.43. The galactokinase (GALL) promoter is a deletion variant of GAL1, which lacks one of the 3 UAS elements required for full induction of the promoter by galactose. The promoter is tightly repressed by glucose and is induced at a moderate level by galactose. Low copy number shuttle expression vector. One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.
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References
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Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736
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Shipping Information
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Distributed: freeze-dried
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