p425 GALL (ATCC® 87339)

Applications: YX-type (expression) shuttle vector  /  Depositors: M Funk

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Designations p425 GALL
Depositors M Funk
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 7.5100002288818360
DESCRIPTION OF VECTOR:
Intact vector size: 7.510
Type of vector: plasmid
Cloning sites: SpeI BamHI SmaI PstI HindIII SalI XhoI
Polylinker sites: XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI
Construction: pRS425, GALL promoter
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): LEU2, ->
terminator: CYC1, ->
MCS: SpeI...XhoI, <-
promoter for expression: GALL, <-
marker(s): ampR, <-
replicon: 2 micron, ->
Vector: p425 GALL (plasmid)
Promoters: Promoter for expression GALL
Construction: pRS425, GALL promoter
Marker(s):LEU2,ampR
Construct size (kb): 7.510000228881836
Features: marker(s): LEU2
marker(s): ampR
promoter for expression: GALL
replicon: 2 micron
MCS: SpeI...XhoI
terminator: CYC1
Applications
YX-type (expression) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--7.8; EcoRI--5.4, 2.3; SacI/XbaI--3.9, 3.2.
The galactokinase (GALL) promoter is a deletion variant of GAL1, which lacks one of the 3 UAS elements required for full induction of the promoter by galactose.
The promoter is tightly repressed by glucose and is induced at a moderate level by galactose.
High copy number shuttle expression vector.
One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736

Related Products
component of:ATCC 87670
Shipped frozen
Shipping Information Distributed: freeze-dried
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Attachments Vector map for 87339