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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 7.5100002288818360 DESCRIPTION OF VECTOR:
Intact vector size: 7.510
Type of vector: plasmid
Cloning sites: SpeI BamHI SmaI PstI HindIII SalI XhoI
Polylinker sites: XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI
Construction: pRS425, GALL promoter
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): LEU2, ->
terminator: CYC1, ->
MCS: SpeI...XhoI, <-
promoter for expression: GALL, <-
marker(s): ampR, <-
replicon: 2 micron, -> Vector: p425 GALL (plasmid) Promoters: Promoter for expression GALL Construction: pRS425, GALL promoter Marker(s):LEU2,ampR Construct size (kb): 7.510000228881836 Features: marker(s): LEU2
marker(s): ampR
promoter for expression: GALL
replicon: 2 micron
MCS: SpeI...XhoI
terminator: CYC1
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Applications
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YX-type (expression) shuttle vector
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Comments
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Restriction digests of the clone give the following sizes (kb): BamHI--7.8; EcoRI--5.4, 2.3; SacI/XbaI--3.9, 3.2. The galactokinase (GALL) promoter is a deletion variant of GAL1, which lacks one of the 3 UAS elements required for full induction of the promoter by galactose. The promoter is tightly repressed by glucose and is induced at a moderate level by galactose. High copy number shuttle expression vector. One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.
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References
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Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736
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Shipping Information
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Distributed: freeze-dried
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