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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 3.6500000953674320 Vector: pLDR21 (plasmid) Promoters: Promoter ptac Construction: pT7-7, pJF118EH Marker(s):ampR Construct size (kb): 3.650000095367432 Features: E. coli promoter cassette: lacIq - ptac cassette
marker(s): ampR
operator: lac
promoter: ptac
replicon: pMB1
repressor gene: lacIq
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Applications
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contains easily purifiable cassette(s) for construction lacIq - ptac promoter cassette
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Comments
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Restriction digests of the clone give the following sizes (kb): BglI--4.0; ClaI--4.0; XhoI--2.3, 1.65. Helper plasmid with an XhoI promoter cassette containing the gene for lacIq and the ptac promoter.
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References
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Diederich L, et al. A versatile plasmid vector system for the regulated expression of genes in Escherichia coli. BioTechniques 16: 916-920, 1994. PubMed: 8068348
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