pLDR21 [WM2194] (ATCC® 87206)

Applications: contains easily purifiable cassette(s) for construction lacIq - ptac promoter cassette  /  Depositors: W Messer

Permits and Restrictions

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Designations pLDR21 [WM2194]
Depositors W Messer
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 3.6500000953674320
Vector: pLDR21 (plasmid)
Promoters: Promoter ptac
Construction: pT7-7, pJF118EH
Marker(s):ampR
Construct size (kb): 3.650000095367432
Features: E. coli promoter cassette: lacIq - ptac cassette
marker(s): ampR
operator: lac
promoter: ptac
replicon: pMB1
repressor gene: lacIq
Applications
contains easily purifiable cassette(s) for construction lacIq - ptac promoter cassette
Comments
Restriction digests of the clone give the following sizes (kb): BglI--4.0; ClaI--4.0; XhoI--2.3, 1.65.
Helper plasmid with an XhoI promoter cassette containing the gene for lacIq and the ptac promoter.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Diederich L, et al. A versatile plasmid vector system for the regulated expression of genes in Escherichia coli. BioTechniques 16: 916-920, 1994. PubMed: 8068348

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map