|
Permits
|
These permits may be required for shipping this product:
-
Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
|
|
Vector Information
|
Size (kb): 3.2170000076293950 Vector: pLDR20 (plasmid) Construction: pOU71, pT7-7 Marker(s):ampR Construct size (kb): 3.217000007629395 Features: E. coli promoter cassette: cI857 - PR cassette
marker(s): ampR
replicon: pMB1
repressor gene: cI857
MCS: ClaI...XhoI
|
|
Applications
|
contains easily purifiable cassette(s) for construction cI857 - PR cassette
|
|
Comments
|
Restriction digests of the clone give the following sizes (kb): BglI--3.35; HindIII--3.4; XhoI--2.1, 1.15. Helper plasmid with an XhoI promoter cassette containing the gene for cI857 and the lambda PR promoter.
|
|
References
|
Diederich L, et al. A versatile plasmid vector system for the regulated expression of genes in Escherichia coli. BioTechniques 16: 916-920, 1994. PubMed: 8068348
|
|
Shipping Information
|
Frozen glycerol stock of E. coli containing the plasmid
|