pLDR20 [WM2193] (ATCC® 87205)

Applications: contains easily purifiable cassette(s) for construction cI857 - PR cassette  /  Depositors: W Messer

Permits and Restrictions

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Designations pLDR20 [WM2193]
Depositors W Messer
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 3.2170000076293950
Vector: pLDR20 (plasmid)
Construction: pOU71, pT7-7
Marker(s):ampR
Construct size (kb): 3.217000007629395
Features: E. coli promoter cassette: cI857 - PR cassette
marker(s): ampR
replicon: pMB1
repressor gene: cI857
MCS: ClaI...XhoI
Applications
contains easily purifiable cassette(s) for construction cI857 - PR cassette
Comments
Restriction digests of the clone give the following sizes (kb): BglI--3.35; HindIII--3.4; XhoI--2.1, 1.15.
Helper plasmid with an XhoI promoter cassette containing the gene for cI857 and the lambda PR promoter.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Diederich L, et al. A versatile plasmid vector system for the regulated expression of genes in Escherichia coli. BioTechniques 16: 916-920, 1994. PubMed: 8068348

Shipped frozen
Shipping Information

 Frozen glycerol stock of  E. coli containing the plasmid

Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 87205