pLDR30 [WM2190, pNON11] (ATCC® 87202™)

Applications: contains easily purifiable cassette(s) for construction pMB1 ori - rop cassettevector for other uses pMB1 ori - rop cassette  /  Depositors: W Messer

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These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Designations pLDR30 [WM2190, pNON11]
Depositors W Messer
Biosafety Level 1
Vector Information
Size (kb): 2.1659998893737790
Vector: pLDR30 (plasmid)
Construction: pT7-7
Marker(s):ampR
Construct size (kb): 2.165999889373779
Features: marker(s): ampR
plasmid replicon cassette: pMB1 ori - rop cassette
MCS: ClaI...EcoRI
Applications
contains easily purifiable cassette(s) for construction pMB1 ori - rop cassette
vector for other uses pMB1 ori - rop cassette
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--2.15; EcoRI--2.15; NotI--1.4, 0.85.
Helper plasmid with a NotI cassette containing a replication origin from pBR322.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Diederich L, et al. A versatile plasmid vector system for the regulated expression of genes in Escherichia coli. BioTechniques 16: 916-920, 1994. PubMed: 8068348

Shipped FD
Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map