pLEX5B [WM2284] (ATCC® 87201)

Applications: cloning vector  /  Depositors: W Messer

Permits and Restrictions

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Designations pLEX5B [WM2284]
Depositors W Messer
Biosafety Level 1
Vector Information
Size (kb): 2.6170001029968260
Vector: pLEX5B (plasmid)
Construction: pT7-7, pNM480
Marker(s):ampR
Construct size (kb): 2.617000102996826
Features: marker(s): ampR
replicon: pMB1
MCS: XmnI...HindIII
ribosome-binding site: Shine-Dalgarno
transcription terminator: rrnB T1T2
Applications
cloning vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.6; HindIII--2.6; XmnI--2.6.
Expression vector with efficient prokaryotic expression signals upstream of the XmnI site and rrnB terminators downstream.
Expression requires fusion of a 5'TG of the insert with the A nucleotide of the XmnI site of the vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Diederich L, et al. A versatile plasmid vector system for the regulated expression of genes in Escherichia coli. BioTechniques 16: 916-920, 1994. PubMed: 8068348

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet
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