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Vector Information
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Size (kb): 5.2890000343322750 Vector: pLEX3B (plasmid) Construction: pT7-7, pNM481 Marker(s):ampR Construct size (kb): 5.289000034332275 Features: marker(s): ampR
replicon: pMB1
reporter group: 'lacZ
MCS: XmnI...HindIII
ribosome-binding site: Shine-Dalgarno
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Applications
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expression vector vector permitting construction of fusion proteins beta-galactosidase beta-D-galactosidase
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Comments
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Restriction digests of the clone give the following sizes (kb): EcoRI--5.2; PstI--5.2; XmnI--5.2. Expression vector with efficient prokaryotic expression signals upstream of the XmnI site. Permits generation of a translational fusion with 'lacZ. pLEX1B, pLEX2B, and pLEX3B (ATCC 87198-87200) differ in the reading frame established at the XmnI site. Expression requires fusion of a 5'TG of the insert with the A nucleotide of the XmnI site of the vector.
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References
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Diederich L, et al. A versatile plasmid vector system for the regulated expression of genes in Escherichia coli. BioTechniques 16: 916-920, 1994. PubMed: 8068348
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