pLEX3B [WM2186] (ATCC® 87200)

Applications: expression vectorvector permitting construction of fusion proteins beta-galactosidase beta-D-galactosidase  /  Depositors: W Messer

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Designations pLEX3B [WM2186]
Depositors W Messer
Biosafety Level 1
Vector Information
Size (kb): 5.2890000343322750
Vector: pLEX3B (plasmid)
Construction: pT7-7, pNM481
Marker(s):ampR
Construct size (kb): 5.289000034332275
Features: marker(s): ampR
replicon: pMB1
reporter group: 'lacZ
MCS: XmnI...HindIII
ribosome-binding site: Shine-Dalgarno
Applications
expression vector
vector permitting construction of fusion proteins beta-galactosidase beta-D-galactosidase
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.2; PstI--5.2; XmnI--5.2.
Expression vector with efficient prokaryotic expression signals upstream of the XmnI site. Permits generation of a translational fusion with 'lacZ. pLEX1B, pLEX2B, and pLEX3B (ATCC 87198-87200) differ in the reading frame established at the XmnI site.
Expression requires fusion of a 5'TG of the insert with the A nucleotide of the XmnI site of the vector.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Diederich L, et al. A versatile plasmid vector system for the regulated expression of genes in Escherichia coli. BioTechniques 16: 916-920, 1994. PubMed: 8068348

Shipped freeze-dried
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