pLEX2B [WM2185] (ATCC® 87199)

Applications: expression vector DNA Segment, single copy, expressedvector permitting construction of fusion proteins beta-galactosidase beta-D-galactosidase  /  Depositors: W Messer

Permits and Restrictions

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Designations pLEX2B [WM2185]
Depositors W Messer
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.2909998893737790
Vector: pLEX2B (plasmid)
Construction: pT7-7, pNM480
Marker(s):ampR
Features: marker(s): ampR
replicon: pMB1
reporter group: 'lacZ
MCS: XmnI...HindIII
ribosome-binding site: Shine-Dalgarno
Applications
expression vector DNA Segment, single copy, expressed
vector permitting construction of fusion proteins beta-galactosidase beta-D-galactosidase
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.4; HindIII--5.4; XmnI--5.4.
Expression vector with efficient prokaryotic expression signals upstream of the XmnI site. Permits generation of a translational fusion with 'lacZ. pLEX1B, pLEX2B, and pLEX3B (ATCC 87198-87200) differ in the reading frame established at the XmnI site.
Expression requires fusion of a 5'TG of the insert with the A nucleotide of the XmnI site of the vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Diederich L, et al. A versatile plasmid vector system for the regulated expression of genes in Escherichia coli. BioTechniques 16: 916-920, 1994. PubMed: 8068348

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet
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