|
Permits
|
These permits may be required for shipping this product:
-
Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
|
|
Vector Information
|
Size (kb): 5.2909998893737790 Vector: pLEX1B (plasmid) Construction: pT7-7, pNM480 Marker(s):ampR Construct size (kb): 5.290999889373779 Features: marker(s): ampR
replicon: pMB1
reporter group: 'lacZ
MCS: XmnI...HindIII
ribosome-binding site: Shine-Dalgarno
|
|
Applications
|
expression vector vector permitting construction of fusion proteins beta-galactosidase beta-D-galactosidase
|
|
Comments
|
Restriction digests of the clone give the following sizes (kb): EcoRI--5.3; HindIII--5.3; PstI--5.3; XmnI--5.3. Expression vector with efficient prokaryotic expression signals upstream of the XmnI site. Permits generation of a translational fusion with 'lacZ. pLEX1B, pLEX2B, and pLEX3B (ATCC 87198-87200) differ in the reading frame established at the XmnI site. Expression requires fusion of a 5'TG of the insert with the A nucleotide of the XmnI site of the vector.
|
|
References
|
Diederich L, et al. A versatile plasmid vector system for the regulated expression of genes in Escherichia coli. BioTechniques 16: 916-920, 1994. PubMed: 8068348
|