pLEX1B [WM2184] (ATCC® 87198)

Applications: expression vectorvector permitting construction of fusion proteins beta-galactosidase beta-D-galactosidase  /  Depositors: W Messer

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Designations pLEX1B [WM2184]
Depositors W Messer
Biosafety Level 1
Vector Information
Size (kb): 5.2909998893737790
Vector: pLEX1B (plasmid)
Construction: pT7-7, pNM480
Marker(s):ampR
Construct size (kb): 5.290999889373779
Features: marker(s): ampR
replicon: pMB1
reporter group: 'lacZ
MCS: XmnI...HindIII
ribosome-binding site: Shine-Dalgarno
Applications
expression vector
vector permitting construction of fusion proteins beta-galactosidase beta-D-galactosidase
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.3; HindIII--5.3; PstI--5.3; XmnI--5.3.
Expression vector with efficient prokaryotic expression signals upstream of the XmnI site. Permits generation of a translational fusion with 'lacZ. pLEX1B, pLEX2B, and pLEX3B (ATCC 87198-87200) differ in the reading frame established at the XmnI site.
Expression requires fusion of a 5'TG of the insert with the A nucleotide of the XmnI site of the vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Diederich L, et al. A versatile plasmid vector system for the regulated expression of genes in Escherichia coli. BioTechniques 16: 916-920, 1994. PubMed: 8068348

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet
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