pKSM713 (ATCC® 87159)

Applications: expression vectorvector permitting RNA synthesis in vitrovector permitting production of single-stranded DNAvector permitting visual detection of recombinants  /  Depositors: K Ippen-Ihler, S Maneewannakul

Permits and Restrictions

View Permits

Designations pKSM713
Depositors K Ippen-Ihler, S Maneewannakul
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.0409998893737790
Vector: pKSM713 (phagemid)
Promoters: Promoter for in vitro transcription T7lac
Construction: pKSM710, pSM102
Construct size (kb): 5.040999889373779
Features: insert detection: lacZalpha
marker(s): kanR
promoter for expression: lacUV5
promoter for in vitro transcription: T7lac
replicon: f1
replicon: pMB1
repressor gene: lacIq
MCS: BamHI...EcoRI
terminator: trpA
transcription terminator: T7
transcription terminator: fd
transcription terminator: rrnB
expression vector
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): BamHI--5.0; EcoRI--5.0; PstI--4.5, 0.75.
Vector contains the following restriction sites (approximate kb from nt 1): BamHI--0.34; BglII--3.47; EcoRI--0.39; KpnI--0.36, 5.02; NcoI--0.33, 2.87; PstI--2.58, 3.25; PvuI--0.51; PvuII--0.48, 3.20, 3.56, 3.90, 3.99; SacI--0.38, 5.03; SalI--2.57.
Differs from pKSM714 (ATCC 87160) only in the orientation of the pSM102 fragment containing lacIq, kanR and the trpA terminator.
Expression vector.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C

Maneewannakul S, et al. The pKSM710 vector cassette provides tightly regulated lac and T7lac promoters and strategies for manipulating N-terminal protein sequences. Plasmid 31: 300-307, 1994. PubMed: 8058823

Shipped freeze-dried