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K Ippen-Ihler, S Maneewannakul
Size (kb): 5.0409998893737790
Vector: pKSM713 (phagemid)
Promoters: Promoter for in vitro transcription T7lac
Construction: pKSM710, pSM102
Construct size (kb): 5.040999889373779
Features: insert detection: lacZalpha
promoter for expression: lacUV5
promoter for in vitro transcription: T7lac
repressor gene: lacIq
transcription terminator: T7
transcription terminator: fd
transcription terminator: rrnB
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): BamHI--5.0; EcoRI--5.0; PstI--4.5, 0.75.
Vector contains the following restriction sites (approximate kb from nt 1): BamHI--0.34; BglII--3.47; EcoRI--0.39; KpnI--0.36, 5.02; NcoI--0.33, 2.87; PstI--2.58, 3.25; PvuI--0.51; PvuII--0.48, 3.20, 3.56, 3.90, 3.99; SacI--0.38, 5.03; SalI--2.57.
Differs from pKSM714 (ATCC 87160)
only in the orientation of the pSM102 fragment containing lacIq, kanR and the trpA terminator.
Maneewannakul S, et al. The pKSM710 vector cassette provides tightly regulated lac and T7lac promoters and strategies for manipulating N-terminal protein sequences. Plasmid 31: 300-307, 1994. PubMed: 8058823