pBSL130 (ATCC® 87145)

Applications: contains easily purifiable cassette(s) for construction omega-aadA1  /  Depositors: MF Alexeyev

Permits and Restrictions

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Designations pBSL130
Depositors MF Alexeyev
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Distribution host: Escherichia coli DH5
Vector Information
Size (kb): 5.0349998474121090
Name of vector: sBSL98
Intact vector size: 3.100
Type of vector: phagemid
Vector end: mLuI
Vector end: mLuI
Cloning sites:
Polylinker sites: KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI
SpeI XbaI NotI/EagI BstXI SacII SacI mLuI
Construction: pBluescript
Host range: Escherichia coli
Features (with orientation and position when available):
MCS: KpnI...SacI mLuI, ->
MCS: mLuI KpnI...SacI, ->
replicon: pMB1
marker(s): ampR, <-
replicon: f1, <-
Cross references:
Vector: pBSL130 (phagemid)
Promoters: Promoter for in vitro transcription T7 (phi10)
Construction: pBluescript
Construct size (kb): 5.034999847412109
Features: marker(s): ampR
promoter for in vitro transcription: T7 (phi10)
replicon: f1
replicon: pMB1
MCS: KpnI...SacI MluI
MCS: MluI KpnI...SacI
coding sequence: aadA (spcR, strR)
contains easily purifiable cassette(s) for construction omega-aadA1
Restriction digests of the clone give the following sizes (kb): MluI--3.0, 2.0; EcoRI--2.8, 2.1; BamHI--2.8, 2.1.
Plasmid containing an easily purified spcR (strR) cassette (flanked by direct repeats of the a multiple cloning sequence) for use in construction.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map