pBSL130 (ATCC® 87145)

Applications: contains easily purifiable cassette(s) for construction omega-aadA1  /  Depositors: MF Alexeyev

Permits and Restrictions

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Designations pBSL130
Depositors MF Alexeyev
Biosafety Level 1
Host
Distribution host: Escherichia coli DH5
Vector Information
Size (kb): 5.0349998474121090
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: sBSL98
Intact vector size: 3.100
Type of vector: phagemid
Vector end: mLuI
Vector end: mLuI
Cloning sites:
Polylinker sites: KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI
SpeI XbaI NotI/EagI BstXI SacII SacI mLuI
Construction: pBluescript
Host range: Escherichia coli
Features (with orientation and position when available):
MCS: KpnI...SacI mLuI, ->
MCS: mLuI KpnI...SacI, ->
replicon: pMB1
marker(s): ampR, <-
replicon: f1, <-
Cross references:
Vector: pBSL130 (phagemid)
Promoters: Promoter for in vitro transcription T7 (phi10)
Construction: pBluescript
Marker(s):ampR,spcR,strR
Construct size (kb): 5.034999847412109
Features: marker(s): ampR
promoter for in vitro transcription: T7 (phi10)
replicon: f1
replicon: pMB1
MCS: KpnI...SacI MluI
MCS: MluI KpnI...SacI
coding sequence: aadA (spcR, strR)
Applications
contains easily purifiable cassette(s) for construction omega-aadA1
Comments
Restriction digests of the clone give the following sizes (kb): MluI--3.0, 2.0; EcoRI--2.8, 2.1; BamHI--2.8, 2.1.
Plasmid containing an easily purified spcR (strR) cassette (flanked by direct repeats of the a multiple cloning sequence) for use in construction.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet
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