Size (kb): 4.0510001182556150
Vector: pBSL121 (phagemid)
Promoters: Promoter for in vitro transcription T7 (phi10)
Construct size (kb): 4.051000118255615
Features: marker(s): ampR
promoter for in vitro transcription: T7 (phi10)
MCS: KpnI...SacI MluI
MCS: MluI KpnI...SacI
coding sequence: cat
contains easily purifiable cassette(s) for construction chloramphenicol acetyltransferase
Restriction digests of the clone give the following sizes (kb): MluI--3.1, 1.0; EcoRI--2.9, 1.2; BamHI--2.9, 1.2.
Contains a chloramphenicol acetyltransferase (cat) cassette flanked by multiple cloning sites in tandem orientation.
Plasmid useful for vector construction and generation of marked deletions and insertions in cloned DNA.
The EcoRI site was removed.
Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718