pBSL121 (ATCC® 87143)

Applications: contains easily purifiable cassette(s) for construction chloramphenicol acetyltransferase  /  Depositors: MF Alexeyev

Permits and Restrictions

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Designations pBSL121
Depositors MF Alexeyev
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 4.0510001182556150
Vector: pBSL121 (phagemid)
Promoters: Promoter for in vitro transcription T7 (phi10)
Construction: pBluescript
Construct size (kb): 4.051000118255615
Features: marker(s): ampR
promoter for in vitro transcription: T7 (phi10)
replicon: f1
replicon: pMB1
MCS: KpnI...SacI MluI
MCS: MluI KpnI...SacI
coding sequence: cat
contains easily purifiable cassette(s) for construction chloramphenicol acetyltransferase
Restriction digests of the clone give the following sizes (kb): MluI--3.1, 1.0; EcoRI--2.9, 1.2; BamHI--2.9, 1.2.
Contains a chloramphenicol acetyltransferase (cat) cassette flanked by multiple cloning sites in tandem orientation.
Plasmid useful for vector construction and generation of marked deletions and insertions in cloned DNA.
The EcoRI site was removed.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map