RR2 (ATCC® 87077)

Applications: vector useful for site-directed mutagenesis  /  Depositors: DH Jones

Permits and Restrictions

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Designations RR2
Depositors DH Jones
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 1.9140000343322750
Vector: RR2 (plasmid)
Construction: pUC19
Marker(s):ampR
Construct size (kb): 1.914000034332275
Features: marker(s): ampR
replicon: pMB1
MCS: BssHII...NotI
Applications
vector useful for site-directed mutagenesis
Comments
Restriction digests of the clone give the following sizes (kb): BglII--1.9; BamHI--1.9; XhoI--1.9.
Insert contains the following restriction sites (approximate kb from nt 1): AvaI--0.05, 0.09; BamHI--0.05; BglII--0.04; BssHII--0.01; DraI--0.92, 0.94, 1.63; FspI--1.28; KpnI--0.11; NotI--0.17; SmaI--0.09; SspI--1.86; XbaI--0.10; XhoI--0.05.
Cloning vector containing a reduced number of restriction sites, useful for site-directed mutagenesis of inserts.
Constructed from pUC19 by elimination of many of the restriction endonuclease sites and replacement of nt 2555-640 with a smaller polylinker.
Differs from RR1 (ATCC 87076) and GenBank S70522 by the presence of a unique FspI site (T1280G) and a C1100T transition.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Jones DH, et al. Production of a vector to facilitate DNA mutagenesis and recombination. BioTechniques 16: 694-701, 1994. PubMed: 8024792

Shipped freeze-dried