RR2 (ATCC® 87077)

Applications: vector useful for site-directed mutagenesis  /  Depositors: DH Jones

Permits and Restrictions

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Designations RR2
Depositors DH Jones
Biosafety Level 1
Vector Information
Size (kb): 1.9140000343322750
Vector: RR2 (plasmid)
Construction: pUC19
Marker(s):ampR
Construct size (kb): 1.914000034332275
Features: marker(s): ampR
replicon: pMB1
MCS: BssHII...NotI
Applications
vector useful for site-directed mutagenesis
Comments
Restriction digests of the clone give the following sizes (kb): BglII--1.9; BamHI--1.9; XhoI--1.9.
Insert contains the following restriction sites (approximate kb from nt 1): AvaI--0.05, 0.09; BamHI--0.05; BglII--0.04; BssHII--0.01; DraI--0.92, 0.94, 1.63; FspI--1.28; KpnI--0.11; NotI--0.17; SmaI--0.09; SspI--1.86; XbaI--0.10; XhoI--0.05.
Cloning vector containing a reduced number of restriction sites, useful for site-directed mutagenesis of inserts.
Constructed from pUC19 by elimination of many of the restriction endonuclease sites and replacement of nt 2555-640 with a smaller polylinker.
Differs from RR1 (ATCC 87076) and GenBank S70522 by the presence of a unique FspI site (T1280G) and a C1100T transition.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Jones DH, et al. Production of a vector to facilitate DNA mutagenesis and recombination. BioTechniques 16: 694-701, 1994. PubMed: 8024792

Shipped freeze-dried
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