RR1 (ATCC® 87076)

Clone Type: Clone  /  Depositors: DH Jones

Permits and Restrictions

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Designations RR1
GenBank Number

S70522

Depositors DH Jones
Applications
vector useful for site-directed mutagenesis
Vector
Construct size (kb): 1.914000034332275
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Comments
Restriction digests of the clone give the following sizes (kb): BglII--1.9; XhoI--1.9; BamHI--1.9.
Insert contains the following restriction sites (approximate kb from nt 1): AvaI--0.05, 0.09; BamHI--0.05; BglII--0.04; BssHII--0.01; DraI--0.92, 0.94, 1.63; KpnI--0.11; NotI--0.17; SmaI--0.09; SspI--1.86; XbaI--0.10; XhoI--0.05.
Cloning vector containing a reduced number of restriction sites, useful for site-directed mutagenesis of inserts.
Constructed from pUC19 by elimination of many of the restriction endonuclease sites and replacement of nt 2555-640 with a smaller polylinker.
Differs from RR2 (ATCC 87077) by a T1100C transition and the loss of an FspI restriction site at nt 1280.
References

Jones DH, et al. Production of a vector to facilitate DNA mutagenesis and recombination. BioTechniques 16: 694-701, 1994. PubMed: 8024792

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