RR1 (ATCC® 87076)

Clone Type: Clone  /  Depositors: DH Jones

Designations RR1
GenBank Number

S70522

Depositors DH Jones
Applications
vector useful for site-directed mutagenesis
Vector
Construct size (kb): 1.914000034332275
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): BglII--1.9; XhoI--1.9; BamHI--1.9.
Insert contains the following restriction sites (approximate kb from nt 1): AvaI--0.05, 0.09; BamHI--0.05; BglII--0.04; BssHII--0.01; DraI--0.92, 0.94, 1.63; KpnI--0.11; NotI--0.17; SmaI--0.09; SspI--1.86; XbaI--0.10; XhoI--0.05.
Cloning vector containing a reduced number of restriction sites, useful for site-directed mutagenesis of inserts.
Constructed from pUC19 by elimination of many of the restriction endonuclease sites and replacement of nt 2555-640 with a smaller polylinker.
Differs from RR2 (ATCC 87077) by a T1100C transition and the loss of an FspI restriction site at nt 1280.
References

Jones DH, et al. Production of a vector to facilitate DNA mutagenesis and recombination. BioTechniques 16: 694-701, 1994. PubMed: 8024792

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