|
Permits
|
These permits may be required for shipping this product:
-
Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
|
|
Vector Information
|
Size (kb): 5.7810001373291020 Vector: pXC47 (plasmid) Promoters: Promoter for expression lambda PL Construction: pXC24, oligo cassette Marker(s):ampR Construct size (kb): 5.781000137329102 Features: initiation codon: ATG
marker(s): ampR
marker(s): tetR
promoter for expression: lambda PL
replicon: ROP copy number control
replicon: pMB1
restriction site: 3' sites HindIII...BamHI
restriction site: 5' SwaI...NsiI/NdeI sites
ribosome-binding site: synthetic Shine-Dalgarno
spacer sequence: ATTTAA
transcription terminator: partially deleted lambda terminator
translational enhancer: T7 gene 10
coding sequence: 14-3-3
|
|
Applications
|
expression vector in another host, produces protein vector useful for cloning PCR products
|
|
Comments
|
Restriction digests of the clone give the following sizes (kb): BamHI--5.8; EcoRI--5.8; HindIII--5.8. The vector contains a 0.7 kb bovine cDNA that can be excised using the 5' and 3' cloning sites and be replaced by a gene of interest. The bovine insert may be used as a positive control for expression. One of 12 expression vectors (ATCC 86990-87001) designed to maximize expression from the lambda PL promoter and support cloning of PCR products. The vectors differ in cloning sites and in translational enhancer and initiation sequences. The insert is expressed as a Met-His fusion protein. Sequences cloned into the NsiI/NdeI site are expressed as a Met-His fusion protein, while sequences cloned into the SwaI site can be expressed as an unfused protein (must provide ATG in cloned insert).
|
|
References
|
Cheng X, Patterson TA. Construction and use of lambda PL promoter vectors for direct cloning and high level expression of PCR amplified DNA coding sequences. Nucleic Acids Res. 20: 4591-4598, 1992. PubMed: 1408761
|
|
Shipping Information
|
Freeze dried E. coli containing the plasmid
|