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Size (kb): 5.8369998931884770
Vector: pXC14 (plasmid)
Promoters: Promoter for expression lambda PL
Construction: pJL6, bovine cDNA
Construct size (kb): 5.836999893188477
Features: initiation codon: ATG
promoter for expression: lambda PL
replicon: ROP copy number control
restriction site: 3' sites HindIII...SalI
restriction site: 5' ClaI site
ribosome-binding site: Shine-Dalgarno sequence
spacer sequence: TACTTACAT
transcription terminator: partially deleted lambda R1 terminator
coding sequence: 14-3-3
in another host, produces protein
vector useful for cloning PCR products
Restriction digests of the clone give the following sizes (kb): BamHI--5.8; EcoRI--5.8; HindIII--5.8.
The vector contains a 0.7 kb bovine cDNA that can be excised using the 5' and 3' cloning sites and be replaced by a gene of interest. The bovine insert may be used as a positive control for expression.
One of 12 expression vectors (ATCC 86990-87001) designed to maximize expression from the lambda PL promoter and support cloning of PCR products. The vectors differ in cloning sites and in translational enhancer and initiation sequences.
The bovine insert sequence and ClaI cloning site are out of frame with the lambda cII N-terminal coding sequence, resulting in production of unfused protein from the lambda PL promoter.
Cheng X, Patterson TA. Construction and use of lambda PL promoter vectors for direct cloning and high level expression of PCR amplified DNA coding sequences. Nucleic Acids Res. 20: 4591-4598, 1992. PubMed: 1408761