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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 4.7719998359680180 Vector: pLS88 (plasmid) Marker(s):kanR,strR,sulR Construct size (kb): 4.771999835968018 Features: marker(s): kanR, strR, sulR
replicon: pLS88
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Comments
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Restriction digests of the clone give the following sizes (kb): SmaI--4.8; HindIII--4.8; PvuI--4.8. The sulfonamide resistance gene (sulII) contains PstI and HincII sites. The kanamycin resistance gene contains unique sites for ClaI, HindIII, PvuI, SmaI and XhoI. natural isolate from Haemophilus ducreyi strain CH37 Broad host range plasmid containing three antibiotic resistance markers. Demonstrated to transform (electroporation) Enterobacteriaceae, Pasteurellaceae, and Pseudomonaceae, but not Camplyobacter, Streptococcaceae, or Staphylococcus aureus ( ATCC 25923). The order of the major features of the plasmid is: PstI - P6 promoter - sulII - strA --> - oriV - kanR --> - P5 promoter.
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References
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Scholz P, et al. Complete nucleotide sequence and gene organization of the broad-host-range plasmid RSF1010. Gene 75: 271-288, 1989. PubMed: 2653965
Willson PJ, et al. Characterization of a multiple antibiotic resistance plasmid from Haemophilus ducreyi. Antimicrob. Agents Chemother. 33: 1627-1630, 1989. PubMed: 2684012
Hansen EJ, et al. Use of electroporation to construct isogenic mutants of Haemophilus ducreyi. J. Bacteriol. 174: 5442-5449, 1992. PubMed: 1644771
Dixon LG, et al. An analysis of the complete nucleotide sequence of the Haemophilus ducreyi broad-host-range plasmid pLS88. Plasmid 32: 228-232, 1994. PubMed: 7846148
Oka A, et al. Nucleotide sequence of the kanamycin resistance transposon Tn903. J. Mol. Biol. 147: 217-226, 1981. PubMed: 6270337
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Shipping Information
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Frozen glycerol stock of E. coli containing the plasmid.
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