pLS88 (ATCC® 86980)

Applications: shuttle vector  /  Depositors: WL Albritton

Permits and Restrictions

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Designations pLS88
Depositors WL Albritton
Biosafety Level 1
Vector Information
Size (kb): 4.7719998359680180
Vector: pLS88 (plasmid)
Marker(s):kanR,strR,sulR
Construct size (kb): 4.771999835968018
Features: marker(s): kanR, strR, sulR
replicon: pLS88
Applications
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): SmaI--4.8; HindIII--4.8; PvuI--4.8.
The sulfonamide resistance gene (sulII) contains PstI and HincII sites. The kanamycin resistance gene contains unique sites for ClaI, HindIII, PvuI, SmaI and XhoI.
natural isolate from Haemophilus ducreyi strain CH37
Broad host range plasmid containing three antibiotic resistance markers.
Demonstrated to transform (electroporation) Enterobacteriaceae, Pasteurellaceae, and Pseudomonaceae, but not Camplyobacter, Streptococcaceae, or Staphylococcus aureus (ATCC 25923).
The order of the major features of the plasmid is: PstI - P6 promoter - sulII - strA --> - oriV - kanR --> - P5 promoter.
Media Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References

Scholz P, et al. Complete nucleotide sequence and gene organization of the broad-host-range plasmid RSF1010. Gene 75: 271-288, 1989. PubMed: 2653965

Willson PJ, et al. Characterization of a multiple antibiotic resistance plasmid from Haemophilus ducreyi. Antimicrob. Agents Chemother. 33: 1627-1630, 1989. PubMed: 2684012

Hansen EJ, et al. Use of electroporation to construct isogenic mutants of Haemophilus ducreyi. J. Bacteriol. 174: 5442-5449, 1992. PubMed: 1644771

Dixon LG, et al. An analysis of the complete nucleotide sequence of the Haemophilus ducreyi broad-host-range plasmid pLS88. Plasmid 32: 228-232, 1994. PubMed: 7846148

Oka A, et al. Nucleotide sequence of the kanamycin resistance transposon Tn903. J. Mol. Biol. 147: 217-226, 1981. PubMed: 6270337

Shipped frozen
Shipping Information

 Frozen glycerol stock of  E. coli containing the plasmid.

Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 86980