pJK210 (ATCC® 86957)

Applications: contains sequence dihydroorotaseintegrating vectorshuttle vectorvector permitting positive selection for integrationvector permitting visual detection of recombinants  /  Depositors: JB Keeney

Permits and Restrictions

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Designations pJK210
Depositors JB Keeney
Biosafety Level 1
Vector Information
Size (kb): 5.0339999198913570
Vector: pJK210 (plasmid)
Promoters: Promoter lac
Construction: pJK142
Marker(s):ampR,ura4+
Construct size (kb): 5.033999919891357
Features: insert detection: lacZ'
marker(s): ampR
marker(s): ura4+
promoter: lac
replicon: pMB1
Applications
contains sequence dihydroorotase
integrating vector
shuttle vector
vector permitting positive selection for integration
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.9; EcoRV--4.9; HindIII--2.6, 1.7, 0.8.
Shuttle vector for integrating sequences at ura4. Permits visual detection of recombinants by lacZalpha complementation.
Constructed from pJK142 (ATCC 86955) by inserting the ura4 gene at the NdeI site.
The ura4 gene contains the following unique restriction sites: BsmI BsgI PflMI Bsu36I StuI BbsI AvrII.
The order of the major feature in the plasmid is: ampR - pMB1 ori - 5' lacZ' - SacI/MCS/KpnI - 3' lacZ' - ura4.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Keeney JB, Boeke JD. Efficient targeted integration at leu1-32 and ura4-294 in Schizosaccharomyces pombe. Genetics 136: 849-856, 1994. PubMed: 8005439

Jill B Keeney, personal communication

Related Products
component of:ATCC 86958
component of:ATCC 87686
Shipped frozen
Shipping Information

 Freeze dried Escherichia coli containing the plasmid.

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