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Clostridium acetobutylicum McCoy et al. emend. Keis et al. (ATCC^® 824^™)

Strain Designations: [CCRC 10639, CCUG 42182, DSM 792, IAM 19013, IFO 13948, JCM 1419, KCTC 1790, L.S. McClung 2291, LMG 5710, McCoy and McClung strain W, NCCB 29024, NCCB 84048, NCIMB 8052, VKM B-1787] / Type Strain: yes / Biosafety Level: 1

Clostridium acetobutylicum McCoy et al. emend. Keis et al. ATCC^® 824^™

freeze-dried

For-Profit: $354.00 Non-Profit: $295.00

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Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Deposited As	Granulobacter pectinovorum (Stormer) Beijerinck
Strain Designations	[CCRC 10639, CCUG 42182, DSM 792, IAM 19013, IFO 13948, JCM 1419, KCTC 1790, L.S. McClung 2291, LMG 5710, McCoy and McClung strain W, NCCB 29024, NCCB 84048, NCIMB 8052, VKM B-1787]
Application	Assay of 4-aminobenzoic acid p-aminobenzoic acid Degrades xylan Produces acetone Produces acidolysin Produces autobacteriocin Produces butyl alcohol butanol Produces ethyl alcohol ethanol Produces restriction endonuclease Cac824I Transformation host
Isolation	Plant-derived foodstuff, cornmeal
Type Strain	yes
Biosafety Level	1
Product Format	freeze-dried
Storage Conditions	Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C

Genome Sequenced Strain	Clostridium acetobutylicum ATCC 824, complete genome
Type Strain	yes
Comments	Genome sequenced strain Verification of identity Agglutination

Medium	ATCC^® Medium 2107: Modified Reinforced Clostridial Agar/Broth Medium ATCC^® Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Growth Conditions	Temperature: 37°C Atmosphere: Anaerobic

Name of Depositor	ER Weyer
Isolation	Plant-derived foodstuff, cornmeal
References	Lee SF, et al. Xylanolytic activity of Clostridium acetobutylicum. Appl. Environ. Microbiol. 50: 1068-1076, 1985. Soucaille P, Goma G. Acetonobutylic fermentation by Clostridium acetobutylicum ATCC 824: Autobacteriocin production, properties, and effects. Curr. Microbiol. 13: 163-169, 1986. Croux C, et al. Purification and characterization of acidolysin, an acidic metalloprotease produced by Clostridium acetobutylicum ATCC 824. Appl. Environ. Microbiol. 56: 3634-3642, 1990. PubMed: 2082818 Validation list no. 51. Int. J. Syst. Bacteriol. 44: 852, 1994. J. Infect. Dis. 56: 333-346, 1935. Mermelstein LD, et al. Expression of cloned homologous fermentative genes in Clostridium acetobutylicum ATCC 824. Bio-Technology 10: 190-195, 1992. PubMed: 1368230 Ash C, et al. Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Antonie van Leeuwenhoek 64: 253-260, 1993. PubMed: 8085788 Wilkinson SR, et al. Phenotypic and genotypic differences between certain strains of Clostridium acetobutylicum. FEMS Microbiol. Lett. 125: 199-204, 1995. Boynton ZL, et al. Cloning, sequencing, and expression of clustered genes encoding beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase, crotonase, and butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824. J. Bacteriol. 178: 3015-3024, 1996. PubMed: 8655474 Boynton ZL, et al. Cloning, sequencing, and expression of genes encoding phosphotransacetylase and acetate kinase from Clostridium acetobutlicum ATCC 824. Appl. Environ. Microbiol. 62: 2758-2766, 1996. PubMed: 8702268 Skerman VB, et al. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30: 225-420, 1980. Girdwood RH. The microbiological assay of vitamins and amino acids. J. Med. Lab. Technol. 20: 26-33, 1963. PubMed: 13948188 Lopez-Contreras AM, et al. Utilisation of saccharides in extruded domestic organic waste by Clostridium acetobutylicum ATCC 824 for production of acetone, butanol and ethanol. Appl. Microbiol. Biotechnol. 54: 162-167, 2000. PubMed: 10968627 Keis S, et al. Emended descriptions of Clostridium acetobutylicum and Clostridium beijerinckii, and descriptions of Clostridium saccharoperbutylacetonicum sp. nov. and Clostridium saccharobutylicum sp. nov.. Int. J. Syst. Evol. Microbiol. 51: 2095-2103, 2001. PubMed: 11760952 Bryant DL, Blaschek HP. Buffering as a means for increasing growth and butanol production by Clostridium acetobutylicum. J. Ind. Microbiol. 3: 49-55, 1988.
Cross References	Nucleotide (GenBank) : X78070 C. acetobutylicum (ATCC 824, T) 16S rRNA Nucleotide (GenBank) : X70334 C. acetobutylicum gene for starch degrading enzyme Nucleotide (GenBank) : M68865 Clostridium acetobutylicum autolytic lysozyme (lyc) gene, complete cds Nucleotide (GenBank) : AF205034 Clostridium acetobutylicum sucrose utilization operon, complete sequence. Nucleotide (GenBank) : M96945 Clostridium acetobutylicum butanol dehydrogenase isozyme A gene, complete cds Nucleotide (GenBank) : M96946 Clostridium acetobutylicum butanol dehydrogenase isozyme B gene, complete cds Nucleotide (GenBank) : AE001438 Clostridium acetobutylicum ATCC 824 megaplasmid pSOL1, complete sequence Nucleotide (GenBank) : S77333 thl=thiolase {5' region} [Clostridium acetobutylicum, ATCC 824, Genomic, 81 nt] Nucleotide (GenBank) : U16166 Clostridium acetobutylicum ATCC 824 16S ribosomal RNA (rrn) gene, partial sequence Nucleotide (GenBank) : AF047822 Clostridium acetobutylicum catabolic acetolactate synthase (alsS) gene, complete cds Nucleotide (GenBank) : U38234 Clostridium acetobutylicum phosphotransacetylase (pta) and acetate kinase (ack) genes, complete cds Nucleotide (GenBank) : AF321779 Clostridium acetobutylicum plasmid pSOL1 aldehyde/alcohol dehydrogenase (adhE2) gene, complete cds Nucleotide (GenBank) : L14744 Clostridium acetobutylicum phosphotransbutyrylase, complete cds; butyrate kinase, complete cds and ORF1 Nucleotide (GenBank) : L14817 Clostridium acetobutylicum transcriptional repressor (solR) and aldehyde-alcohol dehydrogenase (aad) genes, complete cds Nucleotide (GenBank) : U52366 Clostridium acetobutylicum phosphofructokinase (pfk) gene, complete cds, and pyruvate kinase homolog (pyk) gene, partial cds Nucleotide (GenBank) : M93363 Clostridium acetobutylicum acetoacetate decarboxylase, acetoacetyl-coenzyme A:acetate/butyrate:coenzyme A-transferase subunit, complete cds Nucleotide (GenBank) : U08465 Clostridium acetobutylicum ATCC 824 acetyl coenzyme A acetyltransferase gene, complete cds, and homolog of B. subtilis orfX gene, partial cds Nucleotide (GenBank) : U15277 Clostridium acetobutylicum putative regulatory protein and putative ortho phosphoribosyl transferase (pyrE) genes, partial cds and hydrogenase I (hydA) gene, complete cds Nucleotide (GenBank) : U52368 Clostridium acetobutylicum rubredoxin oxidoreductase homolog and deformylase (def) genes, complete cds, and methionyl-tRNA formyltransferase homolog (fmt) gene, partial cds Nucleotide (GenBank) : U07420 Clostridium acetobutylicum ATCC 824 putative sigma factor E (sigE) gene, putative sigma factor E processing enzyme (ORFA), gene and putative sigma factor G (sigG) gene, complete cds Nucleotide (GenBank) : U52367 Clostridium acetobutylicum ATCC 824 F-type ATP synthase subunit a (atpA) gene, F-type ATP synthase subunit c (atpC) gene, and F-type ATP synthase subunit b (atpB) gene, complete cds Nucleotide (GenBank) : U17110 Clostridium acetobutylicum crotonase (crt), putative butyryl-CoA dehydrogenase (BCD), putative a-subunit of electron-transfer flavoprotein (etfA), putative b-subunit of electron-transfer flavoprotein (etfB), and 3-hydroxybutyryl-CoA dehydrogenase Nucleotide (GenBank) : AE001437 Clostridium acetobutylicum ATCC 824, complete genome

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS

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Clostridium acetobutylicum McCoy et al. emend. Keis et al. (ATCC^® 824D-5^™)

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For-Profit: $290.00 Non-Profit: $290.00