pBLCAT6 (ATCC® 77413)

Applications: promoter-cloning vector  /  Depositors: M Boshart

Permits and Restrictions

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Designations pBLCAT6
Depositors M Boshart
Biosafety Level 1
Vector Information
Size (kb): 4.2500000000000000
Vector: pBLCAT6 (plasmid)
Promoters: Promoter none
Construction: pBLCAT3
Marker(s):ampR
Construct size (kb): 4.25
Features: marker(s): ampR
promoter: none
replicon: pMB1
terminator: SV40 polyadenylation
Applications
promoter-cloning vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.6; PvuI--4.3; PvuII--2.5, 1.8; BglI/XhoI--2.9, 1.4; ClaI/XhoI--4.3.
The vector contains the following restriction sites (approximate kb from nt 1): BglI--3.07; ClaI--1.69; EcoRI--0.30, 1.71; HindIII--0.0; NcoI--0.60; PvuI--3.33; PvuII--0.20, 1.89; SacI--1.70; XhoI--0.05.
Promoter-cloning vector for analysis of promoters in vertebrate cells, using the CAT reporter gene.
Constructed from pBLCAT3 (ATCC 37528) by deletion of a 467 bp fragment immediately upstream from the 5' polylinker and replacement by two fragments, each containing the polyadenylation signal of the SV40 large T gene.
The order of the major features in the plasmid is: two SV40 polyadenylation signals - HindIII/MCS/XhoI - cat - SV40 small T splice site - SV40 polyadenylation signal - ClaI/MCS/SstI - pMB1 ori - ampR.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Boshart M, et al. Reporter constructs with low background activity utilizing the cat gene. Gene 110: 129-130, 1992. PubMed: 1544570

Shipped freeze-dried
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