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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 4.2500000000000000 Vector: pBLCAT6 (plasmid) Promoters: Promoter none Construction: pBLCAT3 Marker(s):ampR Construct size (kb): 4.25 Features: marker(s): ampR
promoter: none
replicon: pMB1
terminator: SV40 polyadenylation
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Comments
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Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.6; PvuI--4.3; PvuII--2.5, 1.8; BglI/XhoI--2.9, 1.4; ClaI/XhoI--4.3. The vector contains the following restriction sites (approximate kb from nt 1): BglI--3.07; ClaI--1.69; EcoRI--0.30, 1.71; HindIII--0.0; NcoI--0.60; PvuI--3.33; PvuII--0.20, 1.89; SacI--1.70; XhoI--0.05. Promoter-cloning vector for analysis of promoters in vertebrate cells, using the CAT reporter gene. Constructed from pBLCAT3 ( ATCC 37528) by deletion of a 467 bp fragment immediately upstream from the 5' polylinker and replacement by two fragments, each containing the polyadenylation signal of the SV40 large T gene. The order of the major features in the plasmid is: two SV40 polyadenylation signals - HindIII/MCS/XhoI - cat - SV40 small T splice site - SV40 polyadenylation signal - ClaI/MCS/SstI - pMB1 ori - ampR.
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References
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Boshart M, et al. Reporter constructs with low background activity utilizing the cat gene. Gene 110: 129-130, 1992. PubMed: 1544570
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