pBLCAT6 (ATCC® 77413)

Applications: promoter-cloning vector  /  Depositors: M Boshart

Permits and Restrictions

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Designations pBLCAT6
Depositors M Boshart
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 4.2500000000000000
Vector: pBLCAT6 (plasmid)
Promoters: Promoter none
Construction: pBLCAT3
Marker(s):ampR
Construct size (kb): 4.25
Features: marker(s): ampR
promoter: none
replicon: pMB1
terminator: SV40 polyadenylation
Applications
promoter-cloning vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.6; PvuI--4.3; PvuII--2.5, 1.8; BglI/XhoI--2.9, 1.4; ClaI/XhoI--4.3.
The vector contains the following restriction sites (approximate kb from nt 1): BglI--3.07; ClaI--1.69; EcoRI--0.30, 1.71; HindIII--0.0; NcoI--0.60; PvuI--3.33; PvuII--0.20, 1.89; SacI--1.70; XhoI--0.05.
Promoter-cloning vector for analysis of promoters in vertebrate cells, using the CAT reporter gene.
Constructed from pBLCAT3 (ATCC 37528) by deletion of a 467 bp fragment immediately upstream from the 5' polylinker and replacement by two fragments, each containing the polyadenylation signal of the SV40 large T gene.
The order of the major features in the plasmid is: two SV40 polyadenylation signals - HindIII/MCS/XhoI - cat - SV40 small T splice site - SV40 polyadenylation signal - ClaI/MCS/SstI - pMB1 ori - ampR.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Boshart M, et al. Reporter constructs with low background activity utilizing the cat gene. Gene 110: 129-130, 1992. PubMed: 1544570

Shipped freeze-dried
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